Hutz R J, Dierschke D J, Wolf R C
Biol Reprod. 1986 Feb;34(1):65-70. doi: 10.1095/biolreprod34.1.65.
A new model for the investigation of atresia in rhesus monkeys is presented. This model is based upon the reliable induction of an atresia-like process in the dominant preovulatory follicle (DF) by estradiol-17 beta administered subcutaneously via Silastic capsules for 24 h. Data obtained from follicular contents aspirated from treated animals demonstrated alterations in the putative markers of atresia similar to those described in other models of atresia. Although follicle size and appearance and volume of follicular fluid (FF) were unaltered in treated animals, FF was much more viscous than that aspirated from follicles in untreated animals; this was apparently due to a greater quantity of intercellular matrix that was sensitive to digestion by hyaluronidase. In treated animals, FF concentrations of estrogen (E) and progesterone (P) were depressed 3- and 6.6-fold, respectively. Viability of granulosa cells (GC) from these animals was reduced by 40%, as was their ability to release basal amounts of E and P in vitro. Accumulation of P by GC from treated animals approximated unstimulated control levels when human follicle-stimulating hormone (hFSH) was included in the culture. Therefore, FSH may have a limited capability to "rescue" GC from atresia induced by estradiol. The percentage of cells that bound 125I-hFSH maximally, as measured by autoradiography following 72 h in culture, was not altered by treatment. Oocytes from animals treated with estradiol showed signs of degeneration at aspiration, and deteriorated further in culture. This model is unique in that atresia can be induced in the single DF of a primate species, and thus avoids the disadvantages inherent to studying atresia of heterogeneous follicles in polytocous species.(ABSTRACT TRUNCATED AT 250 WORDS)
本文介绍了一种用于研究恒河猴闭锁的新模型。该模型基于通过硅胶胶囊皮下注射17β - 雌二醇24小时，在优势排卵前卵泡（DF）中可靠诱导出类似闭锁的过程。从经处理动物的卵泡内容物中获取的数据表明，闭锁的假定标志物发生了改变，类似于其他闭锁模型中所描述的情况。虽然经处理动物的卵泡大小、外观和卵泡液（FF）体积未改变，但FF比未处理动物卵泡中吸出的卵泡液粘性大得多；这显然是由于对透明质酸酶消化敏感的细胞间基质数量更多。在经处理动物中，卵泡液中雌激素（E）和孕酮（P）的浓度分别降低了3倍和6.6倍。这些动物颗粒细胞（GC）的活力降低了40%，其在体外释放基础量E和P的能力也降低了。当在培养中加入人卵泡刺激素（hFSH）时，经处理动物的GC对P的积累接近未刺激的对照水平。因此，FSH可能只有有限的能力将GC从雌二醇诱导的闭锁中“挽救”出来。培养72小时后通过放射自显影测量，最大程度结合125I - hFSH的细胞百分比不受处理影响。用雌二醇处理的动物的卵母细胞在吸出时显示出退化迹象，并在培养中进一步恶化。该模型的独特之处在于，可以在灵长类动物的单个DF中诱导闭锁，从而避免了在多胎物种中研究异质性卵泡闭锁所固有的缺点。（摘要截短至250字）
