Cai Fenglin, Xiao Hualin, Sun Yuting, Wang Dongfeng, Tang Jinhai
Department of General Surgery, The Affiliated Cancer Hospital of Nanjing Medical University and Jiangsu Cancer Hospital, and Jiangsu Institute of Cancer Research, 210009, China.
Department of General Surgery, Northern Jiangsu People's Hospital, 225001, China.
J Coll Physicians Surg Pak. 2019 Mar;29(3):240-244. doi: 10.29271/jcpsp.2019.03.240.
To investigate the expression of zinc finger transcription factors-Snail and E-cadherin in adriamycin-resistant human breast cancer MCF-7/ADM cells and non-resistant MCF-7 cells.
An experimental study.
The Affiliated Cancer Hospital of Nanjing Medical University and Jiangsu Cancer Hospital, and Jiangsu Institute of Cancer Research, China, from April 2017 to March 2018.
Real-time quantitative PCR technology was used to detect the expression levels of Snail mRNA and E-cadherin mRNA in normal breast cells, adriamycin-resistant human breast cancer MCF-7/ADM cells and non-resistant MCF-7 cells. Western blot was used to detect the expression levels of proteins of Snail and E-cadherin in normal breast cells, adriamycin-resistant human breast cancer MCF-7/ADM cells and non-resistant MCF-7 cells.
The expression of Snail mRNA and protein in adriamycin-resistant human breast cancer MCF-7/ADM cells was significantly higher than that in normal breast cells (p<0.001) and non-resistant MCF-7 cells (p<0.001). The expression of E-cadherin mRNA and protein in adriamycin-resistant human breast cancer MCF-7/ADM cells was significantly lower than that in normal breast cells (p<0.001) and non-resistant MCF-7 cells (p<0.001).
Adriamycin-resistant human breast cancer MCF-7/ADM cell strains had high expression of Snail and low expression of E-cadherin. This points out to a new research direction for the targeted therapy of drug-resistant breast cancer cells, and provides clinical guidance for breast cancer therapy and prognosis evaluation.
研究锌指转录因子Snail和E-钙黏蛋白在阿霉素耐药的人乳腺癌MCF-7/ADM细胞及非耐药的MCF-7细胞中的表达情况。
实验研究。
中国南京医科大学附属肿瘤医院、江苏省肿瘤医院和江苏省肿瘤研究所，2017年4月至2018年3月。
采用实时定量PCR技术检测正常乳腺细胞、阿霉素耐药的人乳腺癌MCF-7/ADM细胞及非耐药的MCF-7细胞中Snail mRNA和E-钙黏蛋白mRNA的表达水平。采用蛋白质印迹法检测正常乳腺细胞、阿霉素耐药的人乳腺癌MCF-7/ADM细胞及非耐药的MCF-7细胞中Snail和E-钙黏蛋白的蛋白表达水平。
阿霉素耐药的人乳腺癌MCF-7/ADM细胞中Snail mRNA和蛋白的表达显著高于正常乳腺细胞（p<0.001）和非耐药的MCF-7细胞（p<0.001）。阿霉素耐药的人乳腺癌MCF-7/ADM细胞中E-钙黏蛋白mRNA和蛋白的表达显著低于正常乳腺细胞（p<0.001）和非耐药的MCF-7细胞（p<0.001）。
阿霉素耐药的人乳腺癌MCF-7/ADM细胞株Snail高表达，E-钙黏蛋白低表达。这为耐药乳腺癌细胞的靶向治疗指出了新的研究方向，并为乳腺癌治疗及预后评估提供临床指导。
