College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, China.
Carbohydr Polym. 2019 May 1;211:329-335. doi: 10.1016/j.carbpol.2019.02.027. Epub 2019 Feb 10.
Colloidal chitin, the substrate of chitinase with an open hydrated gel-like structure, can be obtained by treatment using either traditional hydrochloric acid (HCl) or ionic liquid (IL) 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]). IL-pretreated chitin provided an efficient production of N-acetylglucosamine (175.62 mg g chitin) and N,N'-diacetylchitobiose (341.70 mg g chitin) with a conversion of 61.49% at 48 h catalyzed by chitinase from Streptomyces albolongus ATCC 27414. A short time second homogenization treatment after IL pretreatment can increase the conversion to 76.11%. A comprehensive characterization and comparison of chitin with different pretreatments suggested that enzymatic performances were correlated with the structural changes (size of the grains and porosity), high decrease in crystallinity, and high enzyme adsorption. The NMR spectroscopy studies of N-acetylglucosamine solvation in [Emim][OAc] clearly suggest that hydrogen bonding is formed between the hydroxyls of N-acetylglucosamine and both the anions and cations of the IL.
胶体几丁质是几丁质酶的基质，具有开放的水合凝胶状结构，可以通过使用传统盐酸（HCl）或离子液体（IL）1-乙基-3-甲基咪唑醋酸盐（[Emim][OAc]）处理来获得。IL 预处理的几丁质在 Streptomyces albolongus ATCC 27414 来源的几丁质酶的催化下，在 48 小时内提供了高效的 N-乙酰葡萄糖胺（175.62 mg g 几丁质）和 N,N'-二乙酰壳二糖（341.70 mg g 几丁质）的生产，转化率为 61.49%。IL 预处理后进行短时间的二次均化处理，可以将转化率提高到 76.11%。对不同预处理方法的几丁质进行全面的表征和比较表明，酶的性能与结构变化（颗粒大小和孔隙率）、结晶度的大幅降低以及高酶吸附有关。N-乙酰葡萄糖胺在[Emim][OAc]中的溶剂化的 NMR 光谱研究清楚地表明，N-乙酰葡萄糖胺的羟基与 IL 的阴离子和阳离子之间形成了氢键。
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