使用N-羟基琥珀酰亚胺（NHS）-荧光素标记抗体。

Labeling Antibodies Using -Hydroxysuccinimide (NHS)-Fluorescein.

作者信息

Berg Eric A, Fishman Jordan B

出版信息

Cold Spring Harb Protoc. 2019 Mar 1;2019(3):2019/3/pdb.prot099283. doi: 10.1101/pdb.prot099283.

Abstract

-Hydroxysuccinimide (NHS)-ester derivatives are among the most commonly used reagents for labeling proteins. The method described here can be adapted to use practically any NHS fluorophore. Generally, a fluorophore is covalently bound to a macromolecule such as an antibody and acts as a reporter molecule used to measure the presence of the macromolecule. These fluorescently labeled bioactive reagents are suitable for use in immunofluorescence, flow cytometry, and numerous other biological applications. There are several widely used dyes available in convenient formats. This protocol can be used with any amine-reactive (e.g., PFP, isothiocyanate) fluorophore derivative.

摘要

N - 羟基琥珀酰亚胺（NHS）酯衍生物是最常用于标记蛋白质的试剂之一。这里描述的方法实际上可以适用于任何NHS荧光团。一般来说，荧光团与诸如抗体等大分子共价结合，并作为用于检测大分子存在的报告分子。这些荧光标记的生物活性试剂适用于免疫荧光、流式细胞术和许多其他生物学应用。有几种格式方便的广泛使用的染料。该方案可用于任何胺反应性（如五氟苯酚、异硫氰酸酯）荧光团衍生物。