Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an City, 271018, P. R. China.
Dong e e Jiao Co., Ltd., Dong'e County, 252200, Shandong Province, P. R. China.
Sci Rep. 2019 Mar 1;9(1):3220. doi: 10.1038/s41598-019-39757-3.
Experiments were conducted to improve jenny conception rates through induced ovulation and timed insemination. Jennies in control, hCG and LH groups were injected intramuscularly with saline alone or saline containing hCG or LH, respectively, when the dominant follicle diameter reached 35 mm. Then, follicle development was checked every 8 h until the dominant follicle ovulated. While 76% of the hCG-treated jennies ovulated between 24 and 48 h, and 84% of the LH-treated ovulated between 24 and 40 h after injection, ovulations in control jennies scattered over an extended period after injection. Conception rates after insemination were significantly higher in LH- or hCG-treated jennies than in the conventionally-bred jennies. The LH preparation used in this study contained more FSH than the hCG preparation did, and supplementing the hCG treatment with FSH significantly improved ovulation synchronization. Ovulations in jennies treated on rainy days were significantly postponed and less synchronized compared to those in jennies treated on sunny days. Together, the results suggested that jenny conception could be significantly improved by inducing ovulation with LH or hCG treatment followed by timed insemination and that FSH and the weather during treatment had profound effects on ovulation induction of jennies.
进行了实验以通过诱导排卵和定时授精来提高母驴受胎率。当优势卵泡直径达到 35mm 时,对照组、hCG 组和 LH 组的母驴分别肌肉注射生理盐水或含有 hCG 或 LH 的生理盐水。然后,每隔 8 小时检查卵泡发育情况,直到优势卵泡排卵。虽然 76%的 hCG 处理母驴在注射后 24 至 48 小时排卵,84%的 LH 处理母驴在注射后 24 至 40 小时排卵,但对照组母驴的排卵时间分散在注射后很长一段时间。LH 或 hCG 处理后的母驴授精后的受胎率明显高于常规繁殖的母驴。本研究中使用的 LH 制剂比 hCG 制剂含有更多的 FSH,并且 hCG 处理中添加 FSH 可显著提高排卵同步性。在雨天处理的母驴的排卵明显延迟,并且与在晴天处理的母驴相比排卵的同步性较差。总之,这些结果表明,通过 LH 或 hCG 处理诱导排卵,然后进行定时授精,可以显著提高母驴的受胎率,并且 FSH 和处理期间的天气对母驴的排卵诱导有深远的影响。