测量凋亡细胞吞噬（胞葬作用）效率。

Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency.

作者信息

Gage Matthew C

机构信息

Division of Medicine, Centre for Cardiometabolic Medicine, University College of London, London, UK.

出版信息

Methods Mol Biol. 2019;1951:143-152. doi: 10.1007/978-1-4939-9130-3_11.

DOI:10.1007/978-1-4939-9130-3_11

PMID:30825150

Abstract

Efferocytosis is the process of recognizing and removing dead and dying cells, performed by a variety of phagocytic cells including macrophages. It has recently been shown that liver X receptor (LXR) signaling in macrophages regulates the expression of important efferocytosis receptors, bridging and signaling molecules. Here we describe a sensitive yet robust efferocytosis assay, optimized to measure bone marrow-derived macrophage (BMDM) apoptotic cell engulfment capability. This assay can be applied to genetically or pharmacologically altered BMDMs.

摘要

胞葬作用是识别和清除死亡及濒死细胞的过程，由包括巨噬细胞在内的多种吞噬细胞执行。最近研究表明，巨噬细胞中的肝脏X受体（LXR）信号传导调节重要胞葬作用受体、桥接分子和信号分子的表达。在此，我们描述了一种灵敏且可靠的胞葬作用检测方法，该方法经过优化以测量骨髓来源巨噬细胞（BMDM）对凋亡细胞的吞噬能力。此检测方法可应用于基因改造或药物处理的BMDM。