• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

组蛋白修饰标记在作为前列腺癌干细胞样细胞模型的前列腺球中强烈调控CDH1启动子。

Histone Modification Marks Strongly Regulate CDH1 Promoter in Prostospheres as A Model of Prostate Cancer Stem Like Cells.

作者信息

Shokraii Fatemeh, Moharrami Maryam, Motamed Nasrin, Shahhoseini Maryam, Totonchi Mehdi, Ezzatizadeh Vahid, Firouzi Javad, Khosravani Pardis, Ebrahimi Marzieh

机构信息

Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran.

Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.

出版信息

Cell J. 2019 Jul;21(2):124-134. doi: 10.22074/cellj.2019.5702. Epub 2019 Feb 20.

DOI:10.22074/cellj.2019.5702
PMID:30825285
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6397603/
Abstract

OBJECTIVE

Cadherin-1 () plays an important role in the metastasis, while expression of this protein is under control of epigenetic changes on its gene promoter. Therefore we evaluated both DNA methylation (DNAmet) and histone modification marks of in prostate cancer stem like cells (PCSLCs).

MATERIALS AND METHODS

In this experimental study, we isolated PCSLCs using cell surface marker and prostaspheroid formation, respectively. The cells isolated from both methods were characterized and then the levels of H3K4me2, H3K27me3, H3K9me2/3 and H3K9ac as well as DNAmet were assessed in promoter of the isolated cells.

RESULTS

The CD44 CD49 cells were not validated as PCSLCs. However, prostaspheres overexpressed stemness related genes and had higher ability of invasion potential, associated with reduction in expression. Epigenetic status analysis showed that promoter was hypo-methylated. Histone modifications of H3K9ac and H3K4me3 were significantly reduced, in parallel with an increased level of H3K27me3.

CONCLUSION

Our results suggest that slight decrease of DNAmet of the CpG island in promoter does not significantly contribute to the change of expression. Therefore, histone modifications are responsible in repressing in PCSLCs.

摘要

目的

钙黏蛋白-1（ ）在转移过程中起重要作用，而该蛋白的表达受其基因启动子表观遗传变化的调控。因此，我们评估了前列腺癌干细胞样细胞（PCSLCs）中 的DNA甲基化（DNAmet）和组蛋白修饰标记。

材料与方法

在本实验研究中，我们分别使用细胞表面标志物和前列腺球形成来分离PCSLCs。对两种方法分离得到的细胞进行表征，然后评估分离细胞 启动子中H3K4me2、H3K27me3、H3K9me2/3和H3K9ac以及DNAmet的水平。

结果

CD44 CD49细胞未被确认为PCSLCs。然而，前列腺球过度表达干性相关基因，具有更高的侵袭潜能，且与 表达降低相关。表观遗传状态分析表明 启动子低甲基化。H3K9ac和H3K4me3的组蛋白修饰显著减少，同时H3K27me3水平升高。

结论

我们的结果表明， 启动子中CpG岛的DNAmet轻微降低对 表达的变化没有显著贡献。因此，组蛋白修饰负责在PCSLCs中抑制 。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b36/6397603/8dc567b3c2bf/Cell-J-21-124-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b36/6397603/4221c47a3450/Cell-J-21-124-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b36/6397603/b391c34cd44b/Cell-J-21-124-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b36/6397603/55812aac9d76/Cell-J-21-124-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b36/6397603/3d2fc6df7406/Cell-J-21-124-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b36/6397603/8dc567b3c2bf/Cell-J-21-124-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b36/6397603/4221c47a3450/Cell-J-21-124-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b36/6397603/b391c34cd44b/Cell-J-21-124-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b36/6397603/55812aac9d76/Cell-J-21-124-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b36/6397603/3d2fc6df7406/Cell-J-21-124-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b36/6397603/8dc567b3c2bf/Cell-J-21-124-g05.jpg

相似文献

1
Histone Modification Marks Strongly Regulate CDH1 Promoter in Prostospheres as A Model of Prostate Cancer Stem Like Cells.组蛋白修饰标记在作为前列腺癌干细胞样细胞模型的前列腺球中强烈调控CDH1启动子。
Cell J. 2019 Jul;21(2):124-134. doi: 10.22074/cellj.2019.5702. Epub 2019 Feb 20.
2
Epigenetic silencing of genes enhanced by collective role of reactive oxygen species and MAPK signaling downstream ERK/Snail axis: Ectopic application of hydrogen peroxide repress CDH1 gene by enhanced DNA methyltransferase activity in human breast cancer.活性氧和 MAPK 信号下游 ERK/Snail 轴的集体作用增强基因的表观遗传沉默：过氧氢气的异位应用通过增强人乳腺癌中的 DNA 甲基转移酶活性来抑制 CDH1 基因。
Biochim Biophys Acta Mol Basis Dis. 2019 Jun 1;1865(6):1651-1665. doi: 10.1016/j.bbadis.2019.04.002. Epub 2019 Apr 4.
3
Heterogeneity in the modification and involvement of chromatin components of the CpG island of the silenced human CDH1 gene in cancer cells.沉默的人类CDH1基因的CpG岛在癌细胞中染色质成分修饰及参与情况的异质性。
Nucleic Acids Res. 2002 Nov 1;30(21):4770-80. doi: 10.1093/nar/gkf593.
4
DNA methylation and not H3K4 trimethylation dictates the expression status of miR-152 gene which inhibits migration of breast cancer cells via DNMT1/CDH1 loop.DNA甲基化而非H3K4三甲基化决定了miR - 152基因的表达状态，该基因通过DNMT1/CDH1环路抑制乳腺癌细胞的迁移。
Exp Cell Res. 2016 Aug 15;346(2):176-87. doi: 10.1016/j.yexcr.2016.07.023. Epub 2016 Jul 28.
5
Genome-wide profiling of histone H3 lysine 9 acetylation and dimethylation in Arabidopsis reveals correlation between multiple histone marks and gene expression.对拟南芥组蛋白 H3 赖氨酸 9 乙酰化和二甲基化的全基因组分析揭示了多个组蛋白标记与基因表达之间的相关性。
Plant Mol Biol. 2010 Apr;72(6):585-95. doi: 10.1007/s11103-009-9594-7.
6
Exposure of Human Prostaspheres to Bisphenol A Epigenetically Regulates SNORD Family Noncoding RNAs via Histone Modification.人前列腺球体暴露于双酚A通过组蛋白修饰对小分子核仁RNA家族非编码RNA进行表观遗传调控。
Endocrinology. 2015 Nov;156(11):3984-95. doi: 10.1210/en.2015-1067. Epub 2015 Aug 6.
7
Recruitment of histone deacetylases HDAC1 and HDAC2 by the transcriptional repressor ZEB1 downregulates E-cadherin expression in pancreatic cancer.转录抑制因子 ZEB1 通过招募组蛋白去乙酰化酶 HDAC1 和 HDAC2 下调胰腺癌中 E-钙黏蛋白的表达。
Gut. 2012 Mar;61(3):439-48. doi: 10.1136/gutjnl-2011-300060. Epub 2011 Dec 5.
8
Chromatin states of developmentally-regulated genes revealed by DNA and histone methylation patterns in zebrafish embryos.斑马鱼胚胎中DNA和组蛋白甲基化模式揭示的发育调控基因的染色质状态
Int J Dev Biol. 2010;54(5):803-13. doi: 10.1387/ijdb.103081ll.
9
E-Cadherin Downregulation is Mediated by Promoter Methylation in Canine Prostate Cancer.E-钙黏蛋白下调由犬前列腺癌中的启动子甲基化介导。
Front Genet. 2019 Nov 29;10:1242. doi: 10.3389/fgene.2019.01242. eCollection 2019.
10
Expression and epigenetic regulation of angiogenesis-related factors during dormancy and recurrent growth of ovarian carcinoma.血管生成相关因子在卵巢癌休眠和复发性生长过程中的表达及其表观遗传调控。
Epigenetics. 2013 Dec;8(12):1330-46. doi: 10.4161/epi.26675. Epub 2013 Oct 17.

引用本文的文献

1
Unlocking the Role of OCT4 in Cancer Lineage Plasticity: A Cross-Cancer Perspective with an Emphasis on Prostate Cancer.揭示OCT4在癌症谱系可塑性中的作用：跨癌症视角，重点关注前列腺癌。
Biomedicines. 2025 Jul 4;13(7):1642. doi: 10.3390/biomedicines13071642.
2
Aspects of the Epigenetic Regulation of EMT Related to Cancer Metastasis.EMT 相关的癌症转移的表观遗传调控方面。
Cells. 2021 Dec 6;10(12):3435. doi: 10.3390/cells10123435.
3
Genetic and Epigenetic Alterations of Regulatory Regions in Hereditary and Sporadic Gastric Cancer.

本文引用的文献

1
Prostate Cancer Stem Cell Markers Drive Progression, Therapeutic Resistance, and Bone Metastasis.前列腺癌干细胞标志物驱动疾病进展、治疗抵抗及骨转移。
Stem Cells Int. 2017;2017:8629234. doi: 10.1155/2017/8629234. Epub 2017 Jun 11.
2
Meta-Analysis of EMT Datasets Reveals Different Types of EMT.上皮-间质转化数据集的荟萃分析揭示了不同类型的上皮-间质转化。
PLoS One. 2016 Jun 3;11(6):e0156839. doi: 10.1371/journal.pone.0156839. eCollection 2016.
3
CD133 does not enrich for the stem cell activity in vivo in adult mouse prostates.CD133在成年小鼠前列腺体内并不能富集干细胞活性。
遗传性和散发性胃癌中调控区域的遗传与表观遗传改变
Pharmaceuticals (Basel). 2021 May 12;14(5):457. doi: 10.3390/ph14050457.
4
Cancer stem cell transcriptome landscape reveals biomarkers driving breast carcinoma heterogeneity.癌症干细胞转录组全景揭示了驱动乳腺癌异质性的生物标志物。
Breast Cancer Res Treat. 2021 Feb;186(1):89-98. doi: 10.1007/s10549-020-06045-y. Epub 2021 Jan 3.
5
Epigenetic Signaling and RNA Regulation in Cardiovascular Diseases.心血管疾病中的表观遗传信号和 RNA 调控。
Int J Mol Sci. 2020 Jan 13;21(2):509. doi: 10.3390/ijms21020509.
Stem Cell Res. 2016 May;16(3):597-606. doi: 10.1016/j.scr.2016.03.003. Epub 2016 Mar 11.
4
Cancer Stem Cell Plasticity Drives Therapeutic Resistance.癌症干细胞可塑性驱动治疗抗性。
Cancers (Basel). 2016 Jan 5;8(1):8. doi: 10.3390/cancers8010008.
5
Cancer stem cells in drug resistant lung cancer: Targeting cell surface markers and signaling pathways.耐药肺癌中的肿瘤干细胞：靶向细胞表面标志物和信号通路。
Pharmacol Ther. 2016 Feb;158:71-90. doi: 10.1016/j.pharmthera.2015.12.001. Epub 2015 Dec 17.
6
Prostate Cancer Stem Cells: Research Advances.前列腺癌干细胞：研究进展
Int J Mol Sci. 2015 Nov 17;16(11):27433-49. doi: 10.3390/ijms161126036.
7
Enrichment of the Cancer Stem Phenotype in Sphere Cultures of Prostate Cancer Cell Lines Occurs through Activation of Developmental Pathways Mediated by the Transcriptional Regulator ΔNp63α.前列腺癌细胞系球状体培养中癌症干细胞表型的富集是通过转录调节因子ΔNp63α介导的发育途径激活而发生的。
PLoS One. 2015 Jun 25;10(6):e0130118. doi: 10.1371/journal.pone.0130118. eCollection 2015.
8
Epigenetic modifications in prostate cancer.前列腺癌中的表观遗传修饰
Epigenomics. 2014;6(4):415-26. doi: 10.2217/epi.14.34.
9
CD44 and CD24 cannot act as cancer stem cell markers in human lung adenocarcinoma cell line A549.CD44和CD24不能作为人肺腺癌细胞系A549中的癌症干细胞标志物。
Cell Mol Biol Lett. 2014 Mar;19(1):23-36. doi: 10.2478/s11658-013-0112-1. Epub 2013 Dec 23.
10
Understanding and targeting cancer stem cells: therapeutic implications and challenges.理解和靶向肿瘤干细胞：治疗意义和挑战。
Acta Pharmacol Sin. 2013 Jun;34(6):732-40. doi: 10.1038/aps.2013.27. Epub 2013 May 20.