具有抗生物膜和再矿化性能的新型生物活性根管封闭剂。

Novel bioactive root canal sealer with antibiofilm and remineralization properties.

机构信息

Department of Advanced Oral Sciences and Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA; Department of Restorative Dental Science, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.

Department of Advanced Oral Sciences and Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA; Department of Operative Dentistry and Endodontics & Stomatology Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

出版信息

J Dent. 2019 Apr;83:67-76. doi: 10.1016/j.jdent.2019.02.006. Epub 2019 Feb 27.

DOI:10.1016/j.jdent.2019.02.006

PMID:30825569

Abstract

OBJECTIVES

(1) To develop a novel bioactive root canal sealer with antibiofilm and remineralization properties using dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP); (2) investigate the effects on E. faecalis biofilm inhibition, sealer flow and sealing ability, compared with an epoxy-resin-based sealer AH Plus; and (3) investigate the calcium (Ca) and phosphate (P) ion release from the sealers.

METHODS

A series of dual-cure endodontic sealers were formulated with DMAHDM and NACP at 5% and 20% by mass, respectively. Flow properties and sealing ability of the sealers were measured. Colony-forming units (CFU), live/dead assay, and polysaccharide production of biofilms on sealers were determined. Ca and P ion releases from the sealers were measured.

RESULTS

The new sealer containing 20% NACP and 5% DMAHDM yielded a paste flow of (28.99 ± 0.69) mm, within the range of ISO recommendations. The sealing properties of the sealer with 5% DMAHDM and 20% NACP were similar to a commercial control (p > 0.05). The sealer with DMAHDM decreased E. faecalis biofilm CFU by more than 4 orders of magnitude, compared to AH plus and experimental controls. The sealer with 20% NACP and 5% DMAHDM had relatively high levels of Ca and P ion release necessary for remineralization.

CONCLUSIONS

A new bioactive endodontic sealer was developed with strong antibiofilm activity against E. faecalis biofilms and high levels of Ca and P ion release for remineralization, without compromising the paste flow and sealing properties.

CLINICAL SIGNIFICANCE

The bioactive antibacterial and remineralizing root canal sealer is promising to inhibit E. faecalis biofilms to prevent endodontic treatment failure and secondary endodontic infections, while releasing high levels of Ca and P ions that could remineralize and strengthen the tooth structures and potentially prevent future root fractures and teeth extractions.

摘要

目的

（1）利用二甲基氨基十六烷基甲基丙烯酸酯（DMAHDM）和无定形磷酸钙纳米颗粒（NACP）开发具有抗生物膜和再矿化性能的新型生物活性根管封闭剂；（2）研究其对粪肠球菌生物膜抑制作用、封闭剂流动和封闭能力的影响，与基于环氧树脂的封闭剂 AH Plus 进行比较；（3）研究封闭剂中钙（Ca）和磷（P）离子的释放情况。

方法

用 DMAHDM 和 NACP 分别以 5%和 20%的质量比制备一系列双固化根管封闭剂。测量封闭剂的流动性能和封闭能力。用封闭剂上生物膜的集落形成单位（CFU）、死活检测和多糖产生来测定生物膜的活性。测量封闭剂中 Ca 和 P 离子的释放情况。

结果

含有 20%NACP 和 5%DMAHDM 的新型封闭剂的糊剂流动度为（28.99±0.69）mm，符合 ISO 建议范围。含 5%DMAHDM 和 20%NACP 的封闭剂的密封性能与商业对照剂相似（p＞0.05）。与 AH plus 和实验对照剂相比，含 DMAHDM 的封闭剂可使粪肠球菌生物膜 CFU 减少超过 4 个数量级。含 20%NACP 和 5%DMAHDM 的封闭剂具有较高水平的 Ca 和 P 离子释放，有利于再矿化。