Department of Biotechnology and Environmental Chemistry, Graduate School of Engineering, Kitami Institute of Technology, 165 Koen-cho, Kitami, Hokkaido 090-8507, Japan.
Department of Biotechnology and Environmental Chemistry, Faculty of Engineering, Kitami Institute of Technology, 165 Koen-cho, Kitami, Hokkaido 090-8507, Japan.
Bioresour Technol. 2019 Jun;281:260-268. doi: 10.1016/j.biortech.2019.02.081. Epub 2019 Feb 23.
Corn cob and corn stover hydrolysates are forms of lignocellulosic biomass that can be used in second generation bioethanol production and biorefinery processes. Growth and fermentation inhibitors generated during physicochemical and enzymatic hydrolysis decrease ethanol and biomaterial production during the subsequent biological processes. Here, estimates of growth and fermentation inhibition during bioethanol fermentation were made using component profiles of corn cobs and corn stover at different degrees of hydrolysis. The component profiles were acquired by non-targeted gas chromatography mass spectrometry and targeted high-performance liquid chromatography. Correlations between the comprehensive analysis results and yeast growth and ethanol production were modeled very accurately by partial-least-squares regression analysis. Acetate, apocynin, butyrovanillone, furfural, furyl hydroxymethyl ketone, m-methoxyacetophenone, palmitic acid, syringaldehyde, and xylose, were compounds with very variable importance in projection values and had negative correlation coefficients in the model. In fact, methoxyacetophenone, apocynin, and syringaldehyde inhibited fermentation more than furfural in equivalent concentration.
玉米芯和玉米秸秆水解物是木质纤维素生物质的一种形式，可用于第二代生物乙醇生产和生物炼制过程。在物理化学和酶水解过程中产生的生长和发酵抑制剂会降低随后的生物过程中乙醇和生物材料的产量。在这里，使用不同水解程度的玉米芯和玉米秸秆的成分谱来估算生物乙醇发酵过程中的生长和发酵抑制。通过非靶向气相色谱质谱和靶向高效液相色谱获得了成分谱。偏最小二乘回归分析非常准确地对综合分析结果与酵母生长和乙醇生产之间的相关性进行了建模。乙酸盐、阿朴肉桂酸、丁基香草醛、糠醛、糠基羟甲基酮、间甲氧基苯乙酮、棕榈酸、丁香醛和木糖是具有非常可变投影值重要性的化合物，在模型中具有负相关系数。事实上，在等效浓度下，甲氧基苯乙酮、阿朴肉桂酸和丁香醛比糠醛对发酵的抑制作用更强。
