School of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China.
School of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China.
Colloids Surf B Biointerfaces. 2019 Jun 1;178:38-43. doi: 10.1016/j.colsurfb.2019.02.046. Epub 2019 Feb 22.
In this study, the interaction between Lycium barbarum polysaccharide (LBP) and unsaturated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) or saturated 1, 2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) was explored using the Langmuir films technique and atomic force microscopy (AFM). Comparing the pure lipid monolayer with the mixed monolayers, the π-A isotherms of the mixed monolayers shifted to larger molecular areas when LBP was added to the subphase. The compression modulus showed that the compressibility of the monolayer films decreased with the addition of LBP. Adsorption curves revealed that the variation in the surface pressure of LBP with POPC was larger than that with DPPC. This phenomenon was verified by the AFM images and the number of each lipid molecule combining with polysaccharide molecules in the mixed monolayer (A value), indicating that hydrophobic interactions between LBP and POPC are stronger than those of DPPC. These findings lay the foundation for exploring the pharmacological mechanism of LBP as an in vivo therapeutic.
本研究采用 Langmuir 膜技术和原子力显微镜（AFM）研究了枸杞多糖（LBP）与不饱和 1-棕榈酰基-2-油酰基-sn-甘油-3-磷酸胆碱（POPC）或饱和 1,2-二棕榈酰基-sn-甘油-3-磷酸胆碱（DPPC）之间的相互作用。与纯脂质单层相比，当 LBP 添加到亚相中时，混合单层的π-A 等温线向更大的分子面积移动。压缩模量表明，随着 LBP 的加入，单层膜的可压缩性降低。吸附曲线表明，LBP 与 POPC 的表面压力变化大于与 DPPC 的变化。这一现象通过 AFM 图像和混合单层中每个脂质分子与多糖分子结合的数量（A 值）得到了验证，表明 LBP 与 POPC 之间的疏水相互作用强于 DPPC。这些发现为探索 LBP 作为体内治疗药物的药理机制奠定了基础。
