核糖体蛋白S3的敲低导致小鼠植入前发育停滞。
Knockdown of ribosomal protein S3 causes preimplantation developmental arrest in mice.
作者信息
Peng Hui, Zhao Yifan, Chen Jing, Huo Jianchao, Zhang Yanyan, Xiao Tianfang
机构信息
College of Animal Science, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002, PR China; University Key Lab for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002, PR China.
College of Animal Science, Fujian Agriculture and Forestry University, Fujian, Fuzhou, 350002, PR China.
出版信息
Theriogenology. 2019 Apr 15;129:77-81. doi: 10.1016/j.theriogenology.2019.02.022. Epub 2019 Feb 23.
Ribosomal protein S3 (RpS3), a member of the ribosome 40S subunit, has conventional ribosomal function and additional extraribosomal functions. The aim of the present study was to analyze the expression and localization of RpS3 and its function in early embryogenesis in mice. RpS3 mRNA and protein were expressed in multiple mouse tissues. In the ovary, RpS3 protein was ubiquitously and highly expressed in oocytes and granulosa cells. After ovulation and fertilization, RpS3 mRNA and protein were detected in oocytes and preimplantation embryos. Furthermore, RpS3 protein was localized in the cytoplasm of oocytes and preimplantation embryos. Moreover, knockdown of RpS3 in zygotes led to a significantly decreased rate of blastocyst formation. These results provide the first evidence for a novel function of RpS3 in regulating early embryonic development in mice.
核糖体蛋白S3（RpS3）是核糖体40S亚基的成员，具有传统的核糖体功能和额外的核糖体外功能。本研究的目的是分析RpS3在小鼠早期胚胎发育中的表达、定位及其功能。RpS3 mRNA和蛋白在小鼠多种组织中表达。在卵巢中，RpS3蛋白在卵母细胞和颗粒细胞中普遍且高表达。排卵和受精后，在卵母细胞和植入前胚胎中检测到RpS3 mRNA和蛋白。此外，RpS3蛋白定位于卵母细胞和植入前胚胎的细胞质中。而且，合子中RpS3的敲低导致囊胚形成率显著降低。这些结果为RpS3在调节小鼠早期胚胎发育中的新功能提供了首个证据。
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