Moore M A, Nakamura T, Shirai T, Ichihara A, Ito N
Jpn J Cancer Res. 1986 Feb;77(2):131-8.
Changes in the level of expression of glucose-6-phosphate dehydrogenase (G6PD) within propyl nitrosamine-induced preneoplastic and neoplastic lesions in F344 rats and Syrian golden hamsters were investigated using an immunohistochemical approach. Previously demonstrated increases in G6PD activity in rat liver and hamster pancreatic foci of altered cells were revealed as being due to elevation in the quantity of enzyme protein, suggesting an underlying change in gene expression. Furthermore, strong positive binding of G6PD antibody in thyroid, lung, urinary bladder and kidney lesions indicated that increase in this enzyme protein might be a common marker for neoplastic alteration, regardless of organ. While the function of elevated G6PD may be related to growth requirements, the finding that preneoplastic lesions in some cases bind more strongly than more malignant populations suggests additional involvement of the enzyme in other biochemical pathway(s) relevant to tumorigenesis.
采用免疫组化方法，研究了丙基亚硝胺诱导的F344大鼠和叙利亚金仓鼠的癌前病变和肿瘤病变中葡萄糖-6-磷酸脱氢酶（G6PD）表达水平的变化。先前已证实在大鼠肝脏和仓鼠胰腺细胞病变灶中G6PD活性增加，这是由于酶蛋白数量增加所致，提示基因表达存在潜在变化。此外，G6PD抗体在甲状腺、肺、膀胱和肾脏病变中呈强阳性结合，表明这种酶蛋白的增加可能是肿瘤性改变的一个常见标志物，与器官无关。虽然G6PD升高的功能可能与生长需求有关，但在某些情况下癌前病变比恶性程度更高的群体结合更强这一发现表明，该酶还参与了与肿瘤发生相关的其他生化途径。
