Joshi Chinmayi, Patel Pooja, Kothari Vijay
Institute of Science, Nirma University, Ahmedabad, Gujarat, 382481, India.
F1000Res. 2019 Jan 18;8:70. doi: 10.12688/f1000research.17430.2. eCollection 2019.
extracts have been prescribed in traditional medicine for management of a variety of disease conditions including microbial infections. Generation of scientific evidence for validation of peel extract's anti-pathogenic efficacy is required. Hydroalcoholic extract of peel (PGPE), prepared by microwave assisted extraction method was evaluated for its quorum-modulatory potential against two different human-pathogenic bacteria viz. and . This extract was able to modulate production of quorum sensing-regulated pigments in both these test bacteria at ≥5 μg/ml. Virulence traits of like haemolytic activity, and biofilm formation were negatively affected by the test extract, and it also made more susceptible to lysis by human serum. Antibiotic susceptibility of both test bacteria was modulated owing to pre-treatment with PGPE. Exposure of these test pathogens to PGPE (≥0.5 μg/ml) effectively reduced their virulence towards the nematode . Repeated subculturing of on PGPE-supplemented growth medium did not induce resistance to PGPE in this notorious pathogen, and this extract was also found to exert a post-extract effect on Individual constituent phytocompounds of PGPE were found to be less efficacious than the whole extract. PGPE seemed to interfere with the machinery of and . PGPE also exhibited notable prebiotic potential by promoting growth of probiotic strains- and at ≤50 μg/ml. This study indicates PGPE to be an effective antipathogenic and prebiotic preparation, and validates its therapeutic use mentioned in traditional medicine. This study also emphasizes the need for testing any bioactive extract at broadest possible concentration range, particularly , so that an accurate picture of dose-response relationship can emerge.
在传统医学中，提取物已被用于治疗包括微生物感染在内的多种疾病。需要生成科学证据来验证果皮提取物的抗病原功效。采用微波辅助提取法制备的果皮水醇提取物（PGPE），针对两种不同的人类病原菌，即[病原菌名称1]和[病原菌名称2]，评估其群体感应调节潜力。该提取物在浓度≥5μg/ml时，能够调节这两种测试细菌中群体感应调节色素的产生。测试提取物对[病原菌名称1]的溶血活性和生物膜形成等毒力特性产生负面影响，并且还使[病原菌名称1]更容易被人血清裂解。由于用PGPE进行预处理，两种测试细菌的抗生素敏感性都受到了调节。将这些测试病原体暴露于PGPE（≥0.5μg/ml）有效地降低了它们对线虫[线虫名称]的毒力。在补充有PGPE的生长培养基上对[病原菌名称1]进行反复传代培养，并未在这种臭名昭著的病原体中诱导出对PGPE的抗性，并且还发现该提取物对[病原菌名称1]具有提取物后效应。发现PGPE的单个植物化学成分的功效不如整个提取物。PGPE似乎干扰了[病原菌名称1]和[病原菌名称2]的[相关机制名称]机制。PGPE在≤50μg/ml时通过促进益生菌菌株[益生菌菌株名称1]和[益生菌菌株名称2]的生长，还表现出显著的益生元潜力。这项研究表明PGPE是一种有效的抗病原和益生元制剂，并验证了其在传统医学中提到的治疗用途。这项研究还强调了需要在尽可能宽的浓度范围内测试任何生物活性提取物，特别是[此处可能有特定说明但未给出具体内容]，以便能够得出准确的剂量反应关系图。
