Gowda D C, Bhavanandan V P, Davidson E A
J Biol Chem. 1986 Apr 15;261(11):4926-34.
The proteoglycans secreted by a malignant human breast cell line (MDA-MB-231) were compared with the corresponding proteoglycans from a normal human breast cell line (HBL-100). The physicochemical characteristics of these proteoglycans were established by hexosamine analysis, chemical and enzymatic degradations, and dissociative cesium chloride density gradient centrifugation, and by gel filtration before and after alkaline beta-elimination. Both cell lines secreted approximately 70% of the synthesized proteoglycans, which were composed of 20% heparan sulfate and 80% chondroitin sulfate proteoglycans. The MDA cell line secreted large hydrodynamic size (major) and small hydrodynamic size heparan sulfate proteoglycan. In contrast HBL cells secreted only one species having a hydrodynamic size intermediate to the above two. The chondroitin sulfate proteoglycans from MDA medium were slightly larger than the corresponding polymers from HBL medium. All proteoglycans except the small hydrodynamic size heparan sulfate proteoglycan from MDA medium were of high buoyant density. The proteoglycans of both cell lines contained significant proportions of disulfide-linked lower molecular weight components which were more pronounced in the proteoheparan sulfate polymers, particularly those from MDA medium, than in chondroitin sulfate proteoglycans. The glycosaminoglycans of heparan sulfate proteoglycans from MDA medium were more heterogeneous than those from HBL medium. The glycosaminoglycan chains of large hydrodynamic size heparan sulfate proteoglycans from MDA medium were larger in size than those from HBL medium while small hydrodynamic size heparan sulfate proteoglycans contained shorter glycosaminoglycan chains. In contrast to the glycosaminoglycans derived from chondroitin sulfate proteoglycans of both MDA and HBL medium were comparable in size. The heparan sulfate as well as chondroitin sulfate proteoglycans of both cell lines contained both neutral (di- and tetrasaccharides) and sialylated (tri- to hexasaccharides) O-linked oligosaccharides.
将恶性人乳腺癌细胞系（MDA-MB-231）分泌的蛋白聚糖与正常人乳腺细胞系（HBL-100）相应的蛋白聚糖进行比较。通过己糖胺分析、化学和酶促降解、解离性氯化铯密度梯度离心以及碱性β-消除前后的凝胶过滤来确定这些蛋白聚糖的物理化学特性。两种细胞系均分泌约70%的合成蛋白聚糖，其由20%的硫酸乙酰肝素和80%的硫酸软骨素蛋白聚糖组成。MDA细胞系分泌大流体力学尺寸（主要）和小流体力学尺寸的硫酸乙酰肝素蛋白聚糖。相比之下，HBL细胞仅分泌一种流体力学尺寸介于上述两者之间的蛋白聚糖。MDA培养基中的硫酸软骨素蛋白聚糖略大于HBL培养基中的相应聚合物。除MDA培养基中的小流体力学尺寸硫酸乙酰肝素蛋白聚糖外，所有蛋白聚糖的浮力密度都很高。两种细胞系的蛋白聚糖都含有相当比例的二硫键连接的低分子量成分，这些成分在硫酸乙酰肝素蛋白聚糖聚合物中比在硫酸软骨素蛋白聚糖中更明显，尤其是来自MDA培养基的那些。MDA培养基中硫酸乙酰肝素蛋白聚糖的糖胺聚糖比HBL培养基中的更具异质性。MDA培养基中大流体力学尺寸硫酸乙酰肝素蛋白聚糖的糖胺聚糖链尺寸比HBL培养基中的大，而小流体力学尺寸硫酸乙酰肝素蛋白聚糖含有较短的糖胺聚糖链。与MDA和HBL培养基中硫酸软骨素蛋白聚糖衍生的糖胺聚糖相比，其尺寸相当。两种细胞系的硫酸乙酰肝素以及硫酸软骨素蛋白聚糖都含有中性（二糖和四糖）和唾液酸化（三糖至六糖）的O-连接寡糖。
