Oil and Plant Protein Center, National Engineering Laboratory for Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, P. R. China.
J Sci Food Agric. 2019 Jul;99(9):4287-4295. doi: 10.1002/jsfa.9661. Epub 2019 Apr 1.
Soybean lecithin powders are good sources of phytosterol glucosides (PGs) containing acyl-sterylglycosides (ASGs) and sterylglucosides (SGs), but PG extraction from soybean lecithin powder is difficult due to the solubilizing property of phospolipids. To comprehensively utilize soybean lecithin resources, an evaluation system construction and factor impact analysis of PG extraction by silica-gel adsorption was investigated in this article.
With high-performance liquid chromatography (HPLC) as the main experimental analysis method, software such as SIMICA and SPSS were applied to construct an evaluation system of PG extraction. Different from scores plot in SIMICA for distinguishing samples in chloroform from others, the loading plot and binary variant correlation analysis of all indicators in PG extraction were brought to confirm four evaluation indicators containing PG purity, ASG recovery, SG recovery and phospholipid recovery. In the factor impact analysis, four times elution from silica-gel sediment was enough to achieve a PG product with least reagent waste, while SPW in petroleum ether at 50 mg mL with 1:3 silica-gel dosage (lecithin/silica-gel, w/w) was then determined as the optimum of single factors.
All studies in this article were of great significance, as they laid foundations for research of PG extraction procedure, as well as PG industrial production, facilitating the comprehensive utilization of lecithin resources. © 2019 Society of Chemical Industry.
大豆卵磷脂粉末是植物甾醇葡萄糖苷（PGs）的良好来源，其中含有酰基甾基糖苷（ASGs）和甾基糖苷（SGs），但由于磷脂的溶解性能，从大豆卵磷脂粉末中提取 PGs 较为困难。为了全面利用大豆卵磷脂资源，本文研究了硅胶吸附法提取 PGs 的评价体系构建及因素影响分析。
以高效液相色谱（HPLC）为主要实验分析方法，应用 SIMICA 和 SPSS 等软件构建了 PG 提取评价体系。与 SIMICA 中用于区分氯仿中样品的得分图不同，PG 提取中的所有指标的加载图和二元变量相关分析被用于确认包含 PG 纯度、ASG 回收率、SG 回收率和磷脂回收率的四个评价指标。在因素影响分析中，硅胶沉积物的四次洗脱足以获得试剂浪费最少的 PG 产物，而 SPW 在 50mg/mL 的石油醚中，硅胶用量（卵磷脂/硅胶，w/w）为 1:3，被确定为单因素的最佳条件。
本文的所有研究都具有重要意义，为 PG 提取工艺的研究以及 PG 的工业生产奠定了基础，有利于大豆卵磷脂资源的综合利用。© 2019 化学工业协会。
