Whiting P J, Vincent A, Schluep M, Newsom-Davis J
J Neuroimmunol. 1986 May;11(3):223-35. doi: 10.1016/0165-5728(86)90006-8.
Ten monoclonal anti-human acetylcholine receptor (AChR) monoclonal antibodies (m.abs) all exhibited high avidity binding to the human AChR. None was able to inhibit alpha-bungarotoxin (alpha-Butx) binding to the receptor. Five distinct but partially overlapping antibody-binding regions were defined by competition experiments. Four antibodies, which competed with each other for one region on denervated human AChR and also bound to human fetal AChR, failed to bind appreciably to normal human AChR in solution, to normal AChR solubilized from 6 other species, or to human endplates in frozen sections.
十种抗人乙酰胆碱受体（AChR）单克隆抗体（m.abs）均表现出与人AChR的高亲和力结合。没有一种能够抑制α-银环蛇毒素（α-Butx）与该受体的结合。通过竞争实验确定了五个不同但部分重叠的抗体结合区域。四种相互竞争去神经化人AChR上一个区域且也与人胎儿AChR结合的抗体，在溶液中未能与正常人AChR、从其他6个物种中溶解的正常AChR或冷冻切片中的人终板明显结合。
