Monteiro Rachel M, Marques Daniella M, Domingues Pedro C A, Oliveira Viviane de C, Macedo Ana Paula, Razaboni Ana M, Watanabe Evandro
Universidade de Sáo Paulo, Escola de Enfermagem de Ribeiráo Preto, Ribeiráo Preto, SP, Brazil.
Universidade de Sáo Paulo, Faculdade de Odontologia de Ribeiráo Preto, Departamento de Materiais Dentários e Próteses, Ribeiráo Preto, SP, Brazil.
Acta Odontol Latinoam. 2018 Dec;31(3):138-143.
Biofilm on dental unit waterlines can spread microbial contamination in the water. The aim of this study was to investigate microbial contamination of water from supplies and dental units before and after the implementation of a protocol for microbial quality improvement and maintenance of dental unit water. The microbial load was evaluated in water from 27 taps and dental units (reservoirs, air-water syringes and highspeed outputs without handpieces) using the PetrifilmT system (total aerobic bacteria and fungi) and conventional culture media (enterobacteria and Legionella spp.). The bacterial load in water samples from taps and reservoirs was within the parameter established by Brazilian legislation (<500CFU/mL); but the bacterial load in samples from air-water syringes and high-speed outputs without handpieces was not. The implementation of the protocol for the maintenance of microbial quality in dental unit water reduced bacterial load in highspeed outputs without handpieces (p=0.004). Enterobacteria and Legionella spp. were not isolated from any of the water samples from taps and dental units.
牙科设备水线中的生物膜会在水中传播微生物污染。本研究的目的是调查在实施改善和维持牙科设备用水微生物质量的方案前后，供水和牙科设备用水中的微生物污染情况。使用PetrifilmT系统（总需氧菌和真菌）和传统培养基（肠杆菌和军团菌属）对来自27个水龙头和牙科设备（储水箱、空气-水注射器和无手机的高速输出口）的水进行微生物负荷评估。来自水龙头和储水箱的水样中的细菌负荷在巴西立法规定的参数范围内（<500CFU/mL）；但来自空气-水注射器和无手机的高速输出口水样中的细菌负荷不在此范围内。实施维持牙科设备用水微生物质量的方案降低了无手机高速输出口中的细菌负荷（p=0.004）。在来自水龙头和牙科设备的任何水样中均未分离出肠杆菌和军团菌属。
