Keskin Serhan, Tatlıpınar Arzu, Ata Pınar, Uzun Selami, Kınal Mustafa Emrah, Erkal Burçin
Ear Nose and Throat Clinic, Health Science University Fatih Sultan Mehmet Health Practice and Research Center, Atasehir, Istanbul, Turkey.
Department of Medical Genetic, Marmara University School of Medicine, Pendik, Istanbul, Turkey.
Eur Arch Otorhinolaryngol. 2019 May;276(5):1321-1325. doi: 10.1007/s00405-019-05366-z. Epub 2019 Mar 4.
Chronic otitis media (COM) is an important debilitating public problem causing hearing loss due to irreversible resorption of the ossicular chain. Activation of osteoprotegerin (OPG) during an acute attack of COM prevents bone resorption.The aim of the study was to investigate the role of OPG gene expression level on ossicular chain resorption in chronic otitis media.
Fifty operated COM patients were included in the study. While 20 patients underwent ossiculoplasty, 30 patients underwent type 1 tympanoplasty. For RNA isolation and OPG gene expression analysis, middle ear swabs were taken from nasopharynx in the ostium of the Eustachian tube. RNA was isolated with mRNA easy kit and kept at - 85 °C till the cDNA and expression analysis. Expression levels were analyzed with real-time quantitative PCR in comparison with PDGB gene expression level as an internal control.
Sample Cq measurements of type 1 tympanoplasty group were higher than Cq measurements of the internal control group (p = 0.027; p < 0.05). In contrast, there was no statistically significant difference between sample Cq measurements of ossiculoplasty group and Cq measurements of the internal control group (p = 0.293; p > 0.05).
Since OPG gene expression level was significantly higher in type 1 tympanoplasty group, OPG gene regulation system may have an effect on ossicular chain destruction in COM.
慢性中耳炎（COM）是一个重要的使人衰弱的公共卫生问题，由于听骨链的不可逆吸收导致听力损失。COM急性发作期间骨保护素（OPG）的激活可防止骨质吸收。本研究的目的是探讨OPG基因表达水平在慢性中耳炎听骨链吸收中的作用。
50例接受手术的COM患者纳入本研究。其中20例患者接受了听骨成形术，30例患者接受了Ⅰ型鼓室成形术。为了进行RNA分离和OPG基因表达分析，在咽鼓管开口处从鼻咽部采集中耳拭子。用mRNA easy试剂盒分离RNA，并保存在-85°C直至进行cDNA和表达分析。与作为内对照的PDGB基因表达水平相比，用实时定量PCR分析表达水平。
Ⅰ型鼓室成形术组样本的Cq测量值高于内对照组的Cq测量值（p = 0.027；p < 0.05）。相比之下，听骨成形术组样本的Cq测量值与内对照组的Cq测量值之间无统计学显著差异（p = 0.293；p > 0.05）。
由于Ⅰ型鼓室成形术组中OPG基因表达水平显著较高，OPG基因调节系统可能对COM中的听骨链破坏有影响。
