Enrichment of dystrophy-associated antigen from erythrocyte membranes of mice with muscular dystrophy.

In a previous report, it was shown that erythrocyte membranes from mice with muscular dystrophy of strain 129/ReJ-dy manifest a unique antigen. In the current study, this antigen was enriched from solubilized membranes by a two-step solid phase immunoadsorbent. The enriched fraction retained antigenicity as well as a reduced set of electrophoretic forms of protein over that seen in solubilized membranes. The enriched fraction contained no novel molecular weight species but several were noticeably enriched, particularly at 54,000 daltons.