O'Donoghue P J, Adams M, Dixon B R, Ford G E, Baverstock P R
J Protozool. 1986 Feb;33(1):114-21. doi: 10.1111/j.1550-7408.1986.tb05569.x.
Isoenzyme electrophoretic techniques were applied to the characterization of seven Sarcocystis spp. that had been identified by conventional morphological studies. Cystozoites were harvested from macroscopic cysts from sheep, cattle, and mice and from microscopic cysts from sheep, cattle, and goats. Soluble cystozoite extracts were subjected to cellulose acetate gel electrophoresis and characterized at 15 of the 39 enzyme loci examined. Genetic relationships among isolates were examined by simple phenetic clustering. Two different morphological types of macroscopic cysts from sheep, identified as S. gigantea (syn. S. ovifelis) and S. medusiformis, consistently differed at 40% of the loci examined. Such genetic divergence confirms their separate morphotypic classification. Both differed from microscopic cyst isolates from sheep at 87% of the loci examined; however, two different morphotypes of microscopic cysts were found in the sheep sampled (thick-walled and thin-walled cysts). Until sufficient numbers of each type can be isolated and examined separately, both were regarded as belonging to the species S. tenella (syn. S. ovicanis). Macroscopic and microscopic cysts from cattle consistently differed at 80% of the loci thereby supporting their separate classification as S. hirsuta (syn. S. bovifelis) and S. cruzi (syn. S. bovicanis), respectively. Isolates from goats (microscopic cysts identified as S. capracanis) differed from S. tenella and S. cruzi at 20% and 47% of the loci, respectively. All macroscopic cyst isolates from the various host animal species (including S. muris from mice) differed from each other at nearly all loci. Isoenzyme electrophoretic techniques therefore provided genetic evidence supporting the classification of these various Sarcocystis spp. by their morphological characteristics.
同工酶电泳技术被应用于对7种通过传统形态学研究已鉴定出的肉孢子虫属物种的特征描述。从绵羊、牛和小鼠的肉眼可见囊肿以及绵羊、牛和山羊的显微镜下可见囊肿中收获包囊体。将可溶性包囊体提取物进行醋酸纤维素凝胶电泳，并在检测的39个酶位点中的15个位点进行特征分析。通过简单的表型聚类来研究分离株之间的遗传关系。来自绵羊的两种不同形态类型的肉眼可见囊肿，被鉴定为巨型肉孢子虫（同义名：绵羊肉孢子虫）和水母状肉孢子虫，在所检测位点的40%上始终存在差异。这种遗传差异证实了它们不同的形态类型分类。两者在所检测位点的87%上与来自绵羊的显微镜下可见囊肿分离株不同；然而，在采样的绵羊中发现了两种不同形态类型的显微镜下可见囊肿（厚壁囊肿和薄壁囊肿）。在能够分别分离并检测到足够数量的每种类型之前，两者都被视为属于柔嫩肉孢子虫（同义名：绵羊犬肉孢子虫）物种。来自牛的肉眼可见囊肿和显微镜下可见囊肿在所检测位点的80%上始终存在差异，从而分别支持将它们分类为刚毛刺肉孢子虫（同义名：牛猫肉孢子虫）和克鲁斯肉孢子虫（同义名：牛犬肉孢子虫）。来自山羊的分离株（显微镜下可见囊肿被鉴定为山羊肉孢子虫）分别在所检测位点的20%和47%上与柔嫩肉孢子虫和克鲁斯肉孢子虫不同。来自各种宿主动物物种的所有肉眼可见囊肿分离株（包括来自小鼠的鼠肉孢子虫）在几乎所有位点上彼此不同。因此，同工酶电泳技术提供了遗传证据，支持根据这些不同肉孢子虫属物种的形态特征进行分类。
