Department of Animal Science, Universidade do Estado de Santa Catarina, Chapecó, RS, Brazil; Graduate Program in Toxicological Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Graduate Program in Toxicological Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Microb Pathog. 2019 May;130:65-70. doi: 10.1016/j.micpath.2019.02.029. Epub 2019 Mar 1.
This study evaluated the seropositivity of Brucella abortus and Leptospira interrogans in ewes with reproductive disturbances in southern Brazil and verified the creatine kinase (CK) activity and oxidation status via assessment of superoxide dismutase, glutathione peroxidase and glutathione transferase in serum of seropositive animals for L. interrogans serovar Icterohaemorrhagiae. For Leptospira infection 381 animals with clinical history of reproductive disturbance from Planalto Serrano de Santa Catarina (Brazil) were analyzed, showing an occurrence for L. interrogans of 20.2% from which 81.8% were seropositive for L. interrogans Icterohaemorrhagiae. Serovars Wolfii, Grippothyphosa, Bratislava, Canicola and Butembo were also identified. In the case of B. abortus, positive cases were identified by buffered acidified antigen, finding 14 positive samples, but none of them were positive after a second test (2-mercaptoethanol), showing the absence of relationship between infection with B. abortus and abortion in the tested individuals. Serum reactive oxygen species (ROS) levels and CK activity were found higher in animals positive for Leptospira infection, presenting higher titrations (1:320) than non-infected individuals. Serum glutathione peroxidase activity was higher in positive animals with titrations 1:160 and 1:320, while serum glutathione S-transferase was higher in positive individuals only for titrations 1:320. Serum superoxide dismutase showed lower activity in infected animals with titrations of 1:320. Our results show the region of Planalto Serrano de Santa Catarina with a high occurrence levels of sheep infected by L. interrogans serovar Icterohaemorrhagiae, from which animals with high titrations (1:320) present oxidative stress elicited by excessive ROS production, triggering the stimulation of antioxidant systems to counter this excess. In summary, ovine with higher titrations (1:320) present oxidative damage that can contribute to disease pathophysiology.
本研究评估了巴西南部繁殖障碍母羊中布鲁氏菌 abortus 和钩端螺旋体 interrogans 的血清阳性率，并通过评估血清中超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽转移酶活性，验证了血清阳性的 icterohaemorrhagiae 血清型钩端螺旋体 interrogans 动物的肌酸激酶 (CK) 活性和氧化状态。为了检测钩端螺旋体感染，对来自圣卡塔琳娜高原塞拉诺（巴西）的 381 头有繁殖障碍临床病史的动物进行了分析，发现钩端螺旋体 interrogans 的发病率为 20.2%，其中 81.8%的动物对钩端螺旋体 interrogans icterohaemorrhagiae 呈血清阳性。还鉴定了沃利氏、 grippothyphosa、布拉迪斯拉发、canicola 和布滕博等血清型。在布鲁氏菌 abortus 的情况下，通过缓冲酸化抗原鉴定出阳性病例，发现 14 个阳性样本，但在第二次测试（2-巯基乙醇）中均未呈阳性，表明在检测个体中，布鲁氏菌 abortus 感染与流产之间没有关系。感染钩端螺旋体的动物血清活性氧 (ROS) 水平和 CK 活性较高，其滴度（1:320）高于未感染个体。阳性动物的血清谷胱甘肽过氧化物酶活性较高，滴度为 1:160 和 1:320，而仅在滴度为 1:320 的阳性个体中，血清谷胱甘肽 S-转移酶活性较高。感染动物的血清超氧化物歧化酶活性较低，滴度为 1:320。我们的研究结果表明，圣卡塔琳娜高原塞拉诺地区的绵羊感染钩端螺旋体 interrogans 血清型 icterohaemorrhagiae 的发生率很高，其中高滴度（1:320）的动物存在由过量 ROS 产生引起的氧化应激，这会刺激抗氧化系统的刺激来对抗这种过量。总之，高滴度（1:320）的绵羊存在氧化损伤，这可能有助于疾病的病理生理学。
