Louie Raymond, Redinbaugh M G, Gordon D T, Abt J J, Anderson R J
USDA-ARS, Corn and Soybean Research, Department of Plant Pathology, The Ohio State University, Wooster 44691.
Department of Plant Pathology, The Ohio State University, Wooster 44691.
Plant Dis. 2000 Oct;84(10):1133-1139. doi: 10.1094/PDIS.2000.84.10.1133.
A new virus was isolated from maize (Zea mays L.) leaves showing mild mosaic symptoms and coinfected with Maize dwarf mosaic virus. The virus was readily transmitted by vascular puncture inoculation (VPI) but not leaf-rub inoculation. Virus symptoms on susceptible maize included pale green, yellow, or cream-colored spots and streaks measuring 1 to 2 mm on emerging leaves 5 to 7 days post-VPI. As leaves developed, the spots and streaks became spindle-shaped, then coalesced into long, chlorotic bands. These bands became translucent and necrotic around the edges. There was a distinctive chlorosis on the stalks that became necrotic. Based on these distinctive symptoms, the new virus was named Maize necrotic streak virus (MNeSV). The virus was not transmitted by Aphis maidis-radicus, Myzus persicae, Macrosiphum euphorbiae, Rhopalosiphum padi, Dalbulus maidis, Graminella nigrifrons, Perigrinus maidis, or Diabrotica virgifera virgifera under persistent or nonpersistent conditions. Both susceptible and resistant maize genotypes were identified following VPI with MNeSV. The isolated virus had isometric (32 nm) virions and a single 29.5-kDa coat protein. MNeSV was serologically distinct from morphologically similar maize viruses. The 4.3-kb single-stranded RNA genome had 25 to 53% sequence identity with species in the family Tombusviridae.
从表现出轻度花叶症状且同时感染玉米矮花叶病毒的玉米（Zea mays L.）叶片中分离出一种新病毒。该病毒可通过维管束穿刺接种（VPI）轻易传播，但不能通过摩擦接种传播。VPI接种后5至7天，易感玉米上的病毒症状包括淡绿色、黄色或奶油色斑点和条纹，出现在新叶上，大小为1至2毫米。随着叶片生长，斑点和条纹变成纺锤形，然后合并成长的褪绿带。这些带在边缘变得半透明并坏死。茎上有明显的褪绿，随后坏死。基于这些独特症状，该新病毒被命名为玉米坏死条纹病毒（MNeSV）。在持久性或非持久性条件下，该病毒不能通过玉米缢管蚜、桃蚜、茄无网蚜、禾谷缢管蚜、玉米叶蝉、黑尾叶蝉、玉米褐飞虱或玉米根萤叶甲传播。用MNeSV进行VPI接种后，鉴定出了易感和抗性玉米基因型。分离出的病毒具有等轴对称（32纳米）的病毒粒子和一种单一的29.5千道尔顿外壳蛋白。MNeSV在血清学上与形态相似的玉米病毒不同。该4.3千碱基单链RNA基因组与番茄病毒科物种的序列同一性为25%至53%。
