Phytopathology. 2002 Nov;92(11):1167-74. doi: 10.1094/PHYTO.2002.92.11.1167.
ABSTRACT A previously uncharacterized virus was isolated from fall-planted sweet corn (Zea mays L., Syngenta GSS 0966) leaves showing fine chlorotic streaks. Symptomatic plants were negative in enzyme-linked immunosorbent assay against many maize viruses, but reacted weakly with antisera to Sorghum stunt mosaic virus suggesting a distant relationship between the viruses. The virus was readily transmitted by vascular puncture inoculation (VPI), but not by leaf-rub inoculation. Symptoms on maize included dwarfing and fine chlorotic streaks along intermediate and small veins that developed 12 to 17 days post-VPI. The isolated virus was bacilliform (231 +/- 5 nm long and 71 +/- 2 nm wide), with a knobby surface, and obvious helical structure typical of rhabdovirus morphology. Nucleorhabdovirus virions were observed by transmission electron microscopy of infected maize leaf tissue sections. Proteins unique to infected plants were observed in extracts of infected leaves, and the isolated virion contained three proteins with molecular masses 82 +/- 2, 50 +/- 3, and 32 +/- 2 kDa. Preliminary sequence analysis indicated the virus had similarity to members of the family Rhabdoviridae. The virus was transmitted by Graminella nigrifrons under persistent conditions. The data indicate the virus, provisionally designated Maize fine streak virus, is a new species in the genus Nucleorhabdovirus.
摘要 从表现出细条斑状褪绿的秋植甜玉米（Zea mays L.，先正达 GSS 0966）叶片中分离到一种以前未鉴定的病毒。在酶联免疫吸附试验中，感病植株对许多玉米病毒呈阴性反应，但与抗高粱矮缩花叶病毒的血清反应较弱，表明这些病毒之间存在远缘关系。该病毒通过叶脉穿刺接种（VPI）很容易传播，但不能通过叶摩擦接种传播。玉米上的症状包括矮化和沿中脉和小脉出现细的褪绿条纹，在 VPI 后 12 至 17 天出现。分离的病毒呈杆状（231 +/- 5nm 长，71 +/- 2nm 宽），表面有结节，具有明显的螺旋结构，典型的弹状病毒形态。感染玉米叶片组织切片的透射电子显微镜观察到核弹状病毒粒子。在感染叶片提取物中观察到感染植物特有的蛋白质，分离的病毒粒子含有三种分子量为 82 +/- 2、50 +/- 3 和 32 +/- 2kDa 的蛋白质。初步序列分析表明，该病毒与弹状病毒科的成员具有相似性。该病毒在持久性条件下通过 Graminella nigrifrons 传播。数据表明，该病毒暂定名为玉米细条斑病毒，是核弹状病毒属的一个新种。
