Bueno Erika, Kisha Ted, Maki Sonja L, von Wettberg Eric J B, Singer Susan
Plant and Soil Science, University of Vermont, Burlington, VT, USA.
USDA ARS, Pullman, WA, USA.
BMC Res Notes. 2019 Mar 4;12(1):117. doi: 10.1186/s13104-019-4152-0.
Chamaecrista fasciculata is a widespread annual legume across Eastern North America, with potential as a restoration planting, biofuel crop, and genetic model for non-papillinoid legumes. As a non-Papilinoid, C. fasciculata, belongs to the Caesalpiniod group in which nodulation likely arose independently of the nodulation in Papilinoid and Mimosoid legumes. Thus, C. fasciculata is an attractive model system for legume evolution. In this study, we describe population structure and genetic diversity among 32 USDA germplasm accessions of C. fasciculata using 317 AFLP markers developed from 12 primer pairs, to assess where geographically there is the most genetic variation.
We found that the C. fasciculata germplasm collection fall into four clusters with admixture among them. After correcting for outliers, our analysis shows two primary groups across Eastern and Central North America. To better understand the population biology of this species, further sampling of the full range of this widespread species is needed across North America, as well as the development of a larger set of markers providing denser coverage of the genome. Further sampling will help clarify geographical relationships in this widespread temperate species.
束花决明是一种广泛分布于北美东部的一年生豆科植物，具有作为恢复种植、生物燃料作物以及非蝶形花亚科豆科植物遗传模型的潜力。作为一种非蝶形花亚科植物，束花决明属于苏木亚科，其结瘤可能独立于蝶形花亚科和含羞草亚科豆科植物的结瘤过程。因此，束花决明是研究豆科植物进化的一个有吸引力的模型系统。在本研究中，我们使用从12对引物开发的317个AFLP标记，描述了32份美国农业部束花决明种质资源的种群结构和遗传多样性，以评估地理上哪里存在最多的遗传变异。
我们发现束花决明种质资源分为四个簇，且它们之间存在混合。在去除异常值后，我们的分析显示在北美东部和中部存在两个主要群体。为了更好地理解该物种的种群生物学，需要在北美对这个广泛分布的物种进行更全面的采样，同时开发一组能更密集覆盖基因组的更多标记。进一步的采样将有助于阐明这个广泛分布的温带物种的地理关系。
