Department of Consumer and Food Sciences, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa; Radiation Technology Centre, Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, P. O. Box LG 80, Legon, Accra, Ghana.
DST/CSIR Nanotechnology Innovation Centre, National Centre for Nano-Structured Materials, Council for Scientific and Industrial Research (CSIR), Meiring Naude Road, Pretoria 0001, South Africa.
Carbohydr Polym. 2019 May 15;212:352-360. doi: 10.1016/j.carbpol.2019.02.065. Epub 2019 Feb 20.
This study determines the effects of stearic acid and gamma irradiation, alone and in combination, on properties of amylose-lipid nanomaterials from pasted high amylose maize starch (HAMS) with and without alpha amylase hydrolysis. HAMS was incorporated with stearic acid (0, 1.5% and 5%, w/w), irradiated at 0, 30 and 60 kGy and pasted under pressure in a rheometer. Isolated materials after thermostable alpha amylase or hot water washing were freeze-dried and characterised using differential scanning calorimetry (DSC), X-ray diffraction (XRD), Atomic Force Microscopy (AFM) and Transmission Electron Microscopy (TEM). The isolated materials contain amylose-lipid complexes (ALCs) as determined by DSC and XRD. Pasting of gamma irradiated HAMS produced type I ALCs, whereas that for un-irradiated HAMS produced type II ALCs. The ALCs occurred at nanoscale with sizes ranging from 10 to 110 nm as observed with AFM and TEM. Tailor-made ALCs nanomaterials can be produced from HAMS (with and without added stearic acid).
本研究旨在确定硬脂酸和γ射线单独及联合作用对糊化高直链玉米淀粉（HAMS）及其经α-淀粉酶水解前后的直链淀粉-脂质纳米材料性能的影响。将 HAMS 与硬脂酸（0、1.5%和 5%，w/w）混合，在 0、30 和 60 kGy 下辐照，然后在流变仪中加压糊化。经热稳定α-淀粉酶或热水洗涤后分离出的材料经冷冻干燥，并采用差示扫描量热法（DSC）、X 射线衍射（XRD）、原子力显微镜（AFM）和透射电子显微镜（TEM）进行表征。通过 DSC 和 XRD 确定分离出的材料中含有直链淀粉-脂质复合物（ALCs）。γ辐照 HAMS 的糊化产生了 I 型 ALCs，而未经辐照的 HAMS 糊化则产生了 II 型 ALCs。通过 AFM 和 TEM 观察到，ALCs 以纳米级存在，尺寸范围为 10 至 110nm。可从 HAMS（添加或不添加硬脂酸）制备定制的 ALCs 纳米材料。
