IBMM, University of Montpellier, CNRS, ENSCM, Montpellier, France.
Faculty of Chemistry, Aalen University, Aalen, Germany.
Anal Chim Acta. 2019 May 30;1057:152-161. doi: 10.1016/j.aca.2019.01.008. Epub 2019 Jan 18.
Successive multiple ionic-polymer layers (SMIL) coatings have been often used in capillary electrophoresis due to their simplicity to implement and regenerate. However, the performances of the separation are strongly dependent on the nature of the polyelectrolyte partners used to build the SMIL coating. In this work, we investigate new couples of polyelectrolytes that were not tested before: namely, polybrene (PB), quaternized diethylaminoethyl dextran (DEAEDq) and ε-poly(lysine) (ε-PLL), as polycations, in combination with poly(acrylic acid), dextran sulfate, poly(styrenesulfonate), poly(methacrylic acid) and poly(l-lysine citramide), as polyanions. Systematic study of intra- and inter-capillaries repeatabilities/reproducibilities was performed based on the determination of migration time, separation efficiency and electroosmotic mobility. Interestingly, the electroosmotic flow was found to vary with the nature of the polycation on a broad range of electroosmotic mobility decreasing in magnitude in the order of PB>ε-PLL > DEAEDq, whatever the polyanion associated. Application of the coatings to the separation of proteins is illustrated in a 0.5 M acetic acid BGE, including CE-MS separation of ribonuclease B-glycoforms of the same mass (positional or structural isomers).
连续离子聚合物层层（SMIL）涂层由于其易于实施和再生而经常用于毛细管电泳。然而，分离性能强烈依赖于用于构建 SMIL 涂层的聚电解质伙伴的性质。在这项工作中，我们研究了以前未测试过的新的聚电解质对：即聚（亚甲基）苯（PB）、季铵化二乙氨基乙基葡聚糖（DEAEDq）和 ε-聚（赖氨酸）（ε-PLL）作为聚阳离子，与聚（丙烯酸）、葡聚糖硫酸盐、聚苯乙烯磺酸盐、聚（甲基丙烯酸）和聚（赖氨酸柠檬酸酰胺）作为聚阴离子结合。基于迁移时间、分离效率和电渗流迁移率的测定，对毛细管内和毛细管间的重复性/重现性进行了系统研究。有趣的是，发现电渗流随聚阳离子的性质而变化，在很大范围内电渗流迁移率降低，顺序为 PB>ε-PLL>DEAEDq，无论与之结合的聚阴离子如何。该涂层在 0.5 M 乙酸 BGE 中的蛋白质分离中的应用包括核糖核酸酶 B 糖型的 CE-MS 分离（质量相同的位置或结构异构体）。
