Department of Chemistry, Chung-Ang University, Seoul, 06974, Republic of Korea.
Sci Rep. 2019 Mar 4;9(1):3348. doi: 10.1038/s41598-019-40169-6.
In this study, a novel resorufin thionocarbonate-based Hg-selective signaling probe (RT) for microfluidic paper-based analytical device (μPAD) applications is reported. The designed probe, RT, was readily synthesized by the one-step reaction of resorufin with phenyl thionochloroformate. The RT probe displayed a prominent color change from yellow to pink and a marked turn-on fluorescence signaling behavior exclusively toward the Hg ion. The signaling of RT was due to Hg-induced hydrolysis of the phenyl thionocarbonate moiety to form the parent resorufin dye, which restored its spectroscopic properties. In addition, RT exhibited the Hg-selective signaling behavior without interference by coexisting environmentally relevant metal ions. The detection limit for Hg in simulated wastewater samples was estimated to be 5.8 × 10 M. In particular, an RT-equipped μPAD prepared using a wax printing technique enabled simple and convenient determination of Hg ions in simulated wastewater samples, with a detection limit of 5.9 × 10 M.
在这项研究中，我们报道了一种新型的基于 Resorufin 硫代碳酸盐的汞选择性信号探针（RT），可用于微流控纸基分析装置（μPAD）应用。设计的探针 RT 可通过一步反应，由 Resorufin 与苯基硫代氯甲酸酯快速合成。RT 探针对 Hg 离子表现出明显的颜色从黄色变为粉红色的变化，以及明显的开启荧光信号行为。RT 的信号是由于 Hg 诱导的苯基硫代碳酸酯部分水解，形成母体 Resorufin 染料，从而恢复其光谱特性。此外，RT 表现出对 Hg 的选择性信号行为，不受共存的环境相关金属离子的干扰。在模拟废水样品中，Hg 的检测限估计为 5.8×10 M。特别地，使用蜡印技术制备的配备 RT 的 μPAD 能够简单方便地测定模拟废水样品中的 Hg 离子，检测限为 5.9×10 M。
