Nie Yue-Hua, Liu Xiao-Dan, Huang Ruixue, Xie Da-Fei, Yin Wen-Jun, Guan Hua, Yu Zi-Jian, Zhou Ping-Kun
Department of Tumor Radiotherapy, The First Affiliated Hospital, University of South China, Hengyang, China.
Department of Radiation Biology, Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing, China.
Dose Response. 2019 Feb 26;17(1):1559325819833474. doi: 10.1177/1559325819833474. eCollection 2019 Jan-Mar.
Radiation therapy induces acute and chronic radiological toxicity, in particular hematological toxicity (HT). This study aimed to explore the mechanistic clue and potential predictors at the messenger RNA (mRNA) level.
Peripheral blood was collected from 3 patients with cervical cancer (CC), nasopharynx cancer (NC), and tongue cancer (TC) after the first 2 Gy fraction of radiotherapy (RT). High-throughput sequencing was used to assess mRNA profiles.
Eleven genes, such as ALAS2(5-aminolevulinate synthase), SLC4A1(solute carrier family 4 member 1), (hemoglobin subunit gamma 2), (TNF α-induced protein 3), (period circadian clock 1), (coiled-coil domain containing 136), (chromosome 9 open reading frame 84), (interleukin 1β), (FosB protooncogene), (nuclear receptor subfamily 4), (polymerase family member 15), had overlapping expression changes in all 3 cancers of which 3 (, and ) are suggested as potential predictors for the early diagnosis of HT after RT.
may be useful predictors of HT in patients after RT. Eleven overlapping expression mRNAs among 3 cancers might be potential predictors for early diagnosis of radiation toxicity in patients.
放射治疗会引发急性和慢性放射性毒性，尤其是血液学毒性（HT）。本研究旨在探索信使核糖核酸（mRNA）水平上的机制线索和潜在预测指标。
收集3例宫颈癌（CC）、鼻咽癌（NC）和舌癌（TC）患者在首次接受2 Gy分割放射治疗（RT）后外周血。采用高通量测序评估mRNA谱。
11个基因，如5-氨基酮戊酸合酶（ALAS2）、溶质载体家族4成员1（SLC4A1）、血红蛋白亚基γ2、肿瘤坏死因子α诱导蛋白3、周期昼夜节律钟1、含卷曲螺旋结构域136、9号染色体开放阅读框84、白细胞介素1β、原癌基因FosB、核受体亚家族4、聚合酶家族成员15，在所有3种癌症中均有重叠的表达变化，其中3个基因（、和）被认为是RT后HT早期诊断的潜在预测指标。
可能是RT后患者HT的有用预测指标。3种癌症中11个重叠表达的mRNA可能是患者放射性毒性早期诊断的潜在预测指标。
