Pirmoradi Zeynab, Yadegari Mayam, Moradi Ali, Khojasteh Fatemeh, Zare Mehrjerdi Fatemeh
Neurobiomedical Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Deptartment of Anatomy and Cell Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Iran J Basic Med Sci. 2019 Feb;22(2):154-159. doi: 10.22038/ijbms.2018.31225.7534.
The main goal of the current research was to examine the effects of Berberine (BBR) on apoptotic signaling and hippocampal oxidative stress induced by common carotid artery occlusion.
Chronic cerebral hypoperfusion (CCH) model was created by occluding the two common carotid arteries (two-vessel occlusion [2VO]) permanently. BBR (50 and 100 mg/kg/daily) was intra-gastrically administered to ischemic rats. Neuronal survival was evaluated by Nissl staining. The levels of malondialdehyde (MDA) and antioxidant enzymes, including catalase (CAT) and superoxide dismutase (SOD), along with the activities of caspase 3 were estimated in the hippocampus 2 month after treating the rats with 2VO.
According to findings of the present research, the BBR therapy inhibited the neuro-degeneration of hippocampus. BBR also significantly decreased the amount of MDA and activity of caspase 3 in the hippocampus. Furthermore, the administration of BBR alleviated the lowered activities of SOD and CAT after 2VO surgery.
The antioxidant and antiapoptotic properties of BBR might play important roles in improving functional outcomes and might have significant neuroprotective effects on the CCH damage.
本研究的主要目的是检测小檗碱（BBR）对颈总动脉闭塞诱导的凋亡信号和海马氧化应激的影响。
通过永久性闭塞双侧颈总动脉（双血管闭塞[2VO]）建立慢性脑灌注不足（CCH）模型。将BBR（50和100mg/kg/每日）经胃内给予缺血大鼠。通过尼氏染色评估神经元存活情况。在用2VO处理大鼠2个月后，估计海马中丙二醛（MDA）和抗氧化酶（包括过氧化氢酶[CAT]和超氧化物歧化酶[SOD]）的水平以及半胱天冬酶3的活性。
根据本研究结果，BBR治疗抑制了海马的神经变性。BBR还显著降低了海马中MDA的量和半胱天冬酶3的活性。此外，BBR给药减轻了2VO手术后SOD和CAT活性的降低。
BBR的抗氧化和抗凋亡特性可能在改善功能结局中起重要作用，并且可能对CCH损伤具有显著的神经保护作用。
