Nishi Oumi, Sato Hiroki
Research Fellowship for Young Scientists, Japan Society for the Promotion of Science, Tokyo, Japan.
Forest Entomology Division, Forestry and Forest Products Research Institute, Tsukuba city, Japan.
Mycology. 2018 Sep 25;10(1):22-31. doi: 10.1080/21501203.2018.1524799. eCollection 2019 Mar.
Understanding the abundance and diversity of fungal entomopathogens associated with plant species is critical for improving their field efficacy as microbial insecticides. is a cosmopolitan entomopathogenic fungus, with some species in this genus showing rhizosphere competencies. This study sought to determine the abundance and diversity of spp. in rhizosphere soils of wild plants in a field in Japan. spp. were detected in 76.2% of 151 rhizosphere soil samples collected from 41 plant species using a plating method. The mean density of spp. in rhizosphere soils was 1.2 × 10 colony forming units/g soil [base 10 logarithm of the mean = 4.06 (S.D. = 0.88)]. There was no significant difference in the densities and detection rates between Asteraceae and Poaceae as well as among two aster and one grass species. The fungal isolates were identified as five species, of which was the most frequently detected and abundant species. No significant specific associations were recognised between the isolated spp. and the examined aster and grass species. Our findings demonstrated the high occurrence and abundance of in rhizosphere soils of wild plants at the sampling site irrespective of host plant taxa.
了解与植物物种相关的真菌病原昆虫的丰度和多样性对于提高其作为微生物杀虫剂的田间效果至关重要。 是一种世界性的昆虫病原真菌,该属中的一些物种具有根际竞争能力。本研究旨在确定日本某田地野生植物根际土壤中 spp. 的丰度和多样性。使用平板计数法从41种植物采集的151份根际土壤样本中,76.2%检测到了 spp.。根际土壤中 spp. 的平均密度为1.2×10菌落形成单位/克土壤[平均值的常用对数=4.06(标准差=0.88)]。菊科和禾本科之间以及两种菊科植物和一种禾本科植物之间的密度和检出率没有显著差异。真菌分离物被鉴定为五个物种,其中 是最常检测到且数量最多的物种。分离出的 spp. 与所检测的菊科植物和禾本科植物之间未发现明显的特异性关联。我们的研究结果表明,无论宿主植物分类如何,采样地点野生植物根际土壤中 spp. 的发生率和丰度都很高。