用于客观评估大鼠正畸牙移动诱导疼痛的咬合力测量。

Bite force measurements for objective evaluations of orthodontic tooth movement-induced pain in rats.

机构信息

Department of Orthodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

West China School of Stomatology, Sichuan University, China.

出版信息

Arch Oral Biol. 2019 May;101:1-7. doi: 10.1016/j.archoralbio.2019.02.002. Epub 2019 Feb 4.

DOI:10.1016/j.archoralbio.2019.02.002

PMID:30836256

Abstract

OBJECTIVE

To examine the reliability of bite force for evaluating orthodontic tooth movement-induced pain in rats.

DESIGN

Orthodontic tooth movement-induced pain was induced by mounting springs (40 g) between incisors and ipsilateral molars in male Sprague-Dawley rats. Five group sets of animals were used: for the first group set, 20 rats were randomly divided into a force group (n = 10) and a sham group (n = 10); for the second group set, 20 rats were divided into a 20-g group and a 80-g group; for the third group set, 20 rats were randomly divided into either a CFA group (complete freund's adjuvant) (n = 10) receiving periodontal injections of CFA at baseline or a control group (n = 10) receiving periodontal injections of saline at baseline; for the forth group set, 24 rats were randomly divided into the following four groups: force + saline, control + saline, force + antiNGF and control + NGF (NGF: nerve growth factor). Rats in the fifth group set were used for immunostaining against CGRP. Bite force and bite frequency were measured at baseline (day 0) and following interventions (day 1, day 3, day 5, day 7 and day 14). Two-way ANOVA with repeated measures was used for statistical analysis and a p value less than 0.05 was considered statistical significance.

RESULTS

Our results revealed that bite force was significantly smaller in the force group than in the sham group at all time points (p < .001). As compared to the control group, periodontal injections of CFA significantly decreased bite force on the 3rd day (p < .01). Moreover, bite force was significantly higher in the force + antiNGF group than in the force + saline group (p = .01 < .05) while significantly smaller in the control + NGF group than in the control + saline group (p < .05). Bite force was similar between the force + antiNGF group and the control + saline group (p = .71 > .05) and between the control + NGF group and the force + saline group (p = .58 > .05). Similar results were found for bite frequency.

摘要

目的

探讨咬合力评估正畸牙移动诱导疼痛的可靠性。

设计

在雄性 Sprague-Dawley 大鼠的切牙和同侧磨牙之间安装弹簧（40g）诱导正畸牙移动诱导疼痛。使用五组动物：第一组 20 只大鼠随机分为力组（n=10）和假手术组（n=10）；第二组 20 只大鼠分为 20g 组和 80g 组；第三组 20 只大鼠随机分为完全弗氏佐剂（CFA）组（n=10）和对照组（n=10），分别在基线时接受牙周内 CFA 注射或生理盐水注射；第四组 24 只大鼠随机分为以下四组：力+盐水、对照+盐水、力+抗 NGF 和对照+NGF（NGF：神经生长因子）。第五组大鼠用于 CGRP 免疫染色。在基线（第 0 天）和干预后（第 1、3、5、7 和 14 天）测量咬合力和咬频。采用重复测量的双因素方差分析进行统计分析，p 值小于 0.05 为有统计学意义。

结果

我们的结果表明，在所有时间点，力组的咬合力均明显小于假手术组（p<0.001）。与对照组相比，CFA 牙周注射在第 3 天显著降低了咬合力（p<0.01）。此外，力+抗 NGF 组的咬合力明显高于力+盐水组（p=0.01<0.05），而对照组+NGF 组的咬合力明显低于对照组+盐水组（p<0.05）。力+抗 NGF 组与对照组+盐水组的咬合力相似（p=0.71>0.05），对照组+NGF 组与力+盐水组的咬合力相似（p=0.58>0.05）。咬频也得到了类似的结果。