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亚硝酸盐和山梨酸盐对法兰克福香肠和图林根塞尔维拉特香肠中细菌菌群的影响

Effects of Nitrite and Sorbate on Bacterial Populations in Frankfurters and Thuringer Cervelat.

作者信息

Hallerbach Catherine M, Potter Norman N

机构信息

Department of Food Science, Cornell University, Ithaca, New York 14853.

出版信息

J Food Prot. 1981 May;44(5):341-346. doi: 10.4315/0362-028X-44.5.341.

Abstract

Four batches of frankfurter emulsion were prepared with no additives, 0.26% potassium sorbate, 140 ppm of sodium nitrite plus 550 ppm of sodium isoascorbate, and 40 ppm sodium nitrite plus 0.26% potassium sorbate plus 550 ppm sodium isoascorbate, and processed. Five batches of thuringer cervelat emulsion were prepared with no additives, 0.26% potassium sorbate, 156 ppm of sodium nitrite, 78 ppm of sodium nitrite plus 0.26% potassium sorbate, and 78 ppm of sodium nitrite plus 156 ppm of sodium nitrate, and processed. The finished products were stored aerobically and bacterial growth patterns were monitored. At 20 C, presence of sodium nitrite and potassium sorbate, separately or together, in the frankfurters were without appreciable effect on total aerobic, total anaerobic, gram-positive, and lactobacillus-pediococcus counts, although at 7 to 9 C these additives moderately lowered bacterial counts. Bacterial counts of the thuringer cervelat were not affected by sodium nitrite, potassium sorbate or sodium nitrate at either temperature. Staphylococcus aureus and Clostridium perfringens were inoculated into all emulsions before further processing to determine if the modified cures, or possible changes in normal microflora, influenced these pathogens. S. aureus was reduced to below detectable levels after heat-processing in all systems. C. perfringens survived processing and then underwent equally slow death in all stored frankfurter emulsions, and stabilization of counts in thuringer cervelat emulsions. Results indicate that the modified cures did not appreciably alter the natural microflora of these products, nor survival of added pathogens.

摘要

制备了四批法兰克福香肠乳化物,分别不添加任何添加剂、添加0.26%山梨酸钾、添加140 ppm亚硝酸钠加550 ppm异抗坏血酸钠,以及添加40 ppm亚硝酸钠加0.26%山梨酸钾加550 ppm异抗坏血酸钠,并进行加工处理。制备了五批图林根塞尔维拉特香肠乳化物,分别不添加任何添加剂、添加0.26%山梨酸钾、添加156 ppm亚硝酸钠、添加78 ppm亚硝酸钠加0.26%山梨酸钾,以及添加78 ppm亚硝酸钠加156 ppm硝酸钠,并进行加工处理。将成品进行需氧储存,并监测细菌生长模式。在20℃时,法兰克福香肠中单独或同时存在亚硝酸钠和山梨酸钾,对需氧菌总数、厌氧菌总数、革兰氏阳性菌和乳杆菌-片球菌计数没有明显影响,尽管在7至9℃时,这些添加剂会适度降低细菌计数。在这两个温度下,图林根塞尔维拉特香肠的细菌计数均不受亚硝酸钠、山梨酸钾或硝酸钠的影响。在进一步加工之前,将金黄色葡萄球菌和产气荚膜梭菌接种到所有乳化物中,以确定改良腌制剂或正常微生物群落的可能变化是否会影响这些病原体。在所有系统中,热处理后金黄色葡萄球菌减少到检测水平以下。产气荚膜梭菌在加工后存活下来,然后在所有储存的法兰克福香肠乳化物中以同样缓慢的速度死亡,在图林根塞尔维拉特香肠乳化物中的菌数稳定下来。结果表明,改良腌制剂并未明显改变这些产品的天然微生物群落,也未改变添加病原体的存活情况。

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