[冠心病合并氯吡格雷抵抗患者lncRNA的差异表达]
[Differential expression of lncRNA in patients with coronary artery disease plus clopidogrel resistance].
作者信息
Xie Wenjian, Huang Beibei, Yin Qian, Chen Shaoliang
机构信息
Department of Cardiology, Nanjing Hospital Affiliated to Nanjing Medical University, Nanjing 210006, China.
General Clinical Research Center, Nanjing Hospital Affiliated to Nanjing Medical University, Nanjing 210006, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2019 Jan 28;44(1):9-13. doi: 10.11817/j.issn.1672-7347.2019.01.002.
To explore differential expression of long non-coding RNA (lncRNA) in patients with coronary artery disease plus clopidogrel resistance. Methods: Patients underwent percutaneous coronary intervention (PCI) and treated with clopidogrel were recruited, and their clinical data and blood samples were collected. Patients were divided into a clopidogrel sensitive group and a clopidogrel resistance group according to platelet aggregation rate. lncRNA microarray and real-time RT-PCR were performed in 5 and 34 patients in each group, respectively. Results: lncRNA microarray showed that 11 lncRNAs in peripheral leukocytes were up-regulated and 8 lncRNAs were down-regulated in clopidogrel resistant group. Real-time PCR indicated that two lncRNAs (NONHSAT083775.2 and NONHSAT107804.2) in leukocytes were up-regulated and one lncRNA (NONHSAT133455.2) was down-regulated in the clopidogrel resistant group compared with the clopidogrel sensitive group, consistent with the results of lncRNA microarray. Conclusion: Clopidogrel resistance is associated with the up-regulation of lncRNA NONHSAT083775.2 and NONHSAT107804.2 and the down-regulation of NONHSAT133455.2.
探讨冠心病合并氯吡格雷抵抗患者中长链非编码RNA（lncRNA）的差异表达。方法：招募接受经皮冠状动脉介入治疗（PCI）并使用氯吡格雷治疗的患者，收集其临床资料和血液样本。根据血小板聚集率将患者分为氯吡格雷敏感组和氯吡格雷抵抗组。分别对每组5例和34例患者进行lncRNA芯片和实时逆转录-聚合酶链反应（RT-PCR）检测。结果：lncRNA芯片显示，氯吡格雷抵抗组外周血白细胞中有11种lncRNAs上调，8种lncRNAs下调。实时PCR表明，与氯吡格雷敏感组相比，氯吡格雷抵抗组白细胞中的两种lncRNAs（NONHSAT083775.2和NONHSAT107804.2）上调，一种lncRNA（NONHSAT133455.2）下调，与lncRNA芯片结果一致。结论：氯吡格雷抵抗与lncRNA NONHSAT083775.2和NONHSAT107804.2的上调以及NONHSAT133455.2的下调有关。
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