Idris B M, Dikko A Au, Yarube I U, Salim M A, Saleh M Ia, Alhassan A W
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Niger J Physiol Sci. 2018 Dec 30;33(2):201-209.
Infertility rate is high globally and in Nigeria. The reported spermicidal activity of Citrus aurantifolia juice (CAJ)and its popular consumption may be a contributing factor to the rise in male infertility. This study examined the effects ofCAJ on testis and evaluated the role of calcium and zinc in these effects. Twenty-eight male rats (200-220g) were groupedinto four (n=7). Group I (control) received 0.5ml normal saline, while groups II, III and IV received 600mg/kg, 900mg/kgand 1200mg/kg of CAJ, respectively, orally for 35 days. Sperm analysis, testicular histology, testicular zinc and calciumconcentrations were evaluated. The results showed a significant decrease (P < 0.001) in body weight and gonad-somaticindex (GSI) of the rats in group IV. No sperm cells were found in the sperm samples of all the treatment groups in contrastto control. There was a significant decrease (P < 0.001) in zinc concentration of group III and IV animals and a significantincrease (P < 0.001) in testicular calcium content of group III and IV animals. Derangement of testicular cyto-architecture,shrinkage or complete destruction of seminiferous tubules as well as absence of spermatogenic cells were observed in thetreatment groups. It was concluded that CAJ induced a destructive effect on testes of rats as evidenced by damaged testiculartissue, reduced gonado-somatic index, azospermia and disruption in testicular electrolyte homeostasis. It was concluded thatCAJ caused hypercalcaemia and hypozincaemia in the testicular tissue of the treated rats. Concurrently, CAJ also causeddamage to testicular histology, azospermia and decreased GSI. Citrus aurantifolia juice should be consumed with cautiondue to its potential to cause infertility in males.
全球及尼日利亚的不孕率都很高。据报道，酸橙汁（CAJ）具有杀精活性，且其广泛消费可能是导致男性不孕率上升的一个因素。本研究考察了CAJ对睾丸的影响，并评估了钙和锌在这些影响中的作用。将28只雄性大鼠（200 - 220克）分为四组（每组n = 7）。第一组（对照组）给予0.5毫升生理盐水，而第二、三、四组分别口服600毫克/千克、900毫克/千克和1200毫克/千克的CAJ，持续35天。对精子进行分析、观察睾丸组织学变化、测定睾丸锌和钙的浓度。结果显示，第四组大鼠的体重和性腺 - 体质量指数（GSI）显著下降（P < 0.001）。与对照组相比，所有处理组的精子样本中均未发现精子细胞。第三组和第四组动物的锌浓度显著下降（P < 0.001），而第三组和第四组动物的睾丸钙含量显著增加（P < 0.001）。在处理组中观察到睾丸细胞结构紊乱、生精小管萎缩或完全破坏以及生精细胞缺失。得出的结论是，CAJ对大鼠睾丸产生了破坏作用，表现为睾丸组织受损、性腺 - 体质量指数降低、无精子症以及睾丸电解质稳态紊乱。得出的结论是，CAJ导致处理组大鼠睾丸组织出现高钙血症和低锌血症。同时，CAJ还导致睾丸组织学损伤、无精子症和GSI降低。由于酸橙汁有导致男性不育的潜在风险，应谨慎饮用。
