牙矿化组织中蛋白质原位可视化方法

Methods for In Situ Protein Visualization in Dental Mineralized Tissues.

作者信息

Hotton D, Berdal A, Bolaños A

机构信息

Centre de Recherche des Cordeliers, INSERM UMR_S1138, Equipe Physiopathologie Orale Moléculaire, University Paris-Diderot, Paris, France.

Molecular Oral Pathophysiology, Cordeliers Research Center, UMRS 1138 INSERM, Paris-Descartes, Pierre-et-Marie-Curie, Paris-Diderot Universities, Paris, France.

出版信息

Methods Mol Biol. 2019;1922:173-180. doi: 10.1007/978-1-4939-9012-2_17.

DOI:10.1007/978-1-4939-9012-2_17

PMID:30838575

Abstract

Immunohistochemistry (IHC) is a technique based on the specificity of antibody-antigen principle used commonly to detect antigens in tissue sections. The immune labeling can be performed in paraffin sections, cryostat sections, and ultrathin sections and can be observed in light confocal and transmission electron microscopy. However, the use of immunohistochemical techniques for the study of mineralized tissues has been a challenge for decades (Berdal et al., Arch Oral Biol 36:715-725, 1991; Nanci et al., Eur J Histochem 52:201-214, 2008). Specific procedures are necessary when compared with soft tissue immunohistochemistry. This chapter describes methods for IHC on Tissue-Tek O.C.T. compound and paraffin-embedded sections to detect antigens in the dental mineralized tissues.

摘要

免疫组织化学（IHC）是一种基于抗体 - 抗原特异性原理的技术，常用于检测组织切片中的抗原。免疫标记可在石蜡切片、低温恒温器切片和超薄切片中进行，并可在光学共聚焦显微镜和透射电子显微镜下观察。然而，几十年来，使用免疫组织化学技术研究矿化组织一直是一项挑战（Berdal等人，《口腔生物学文献》36:715 - 725，1991；Nanci等人，《欧洲组织化学杂志》52:201 - 214，2008）。与软组织免疫组织化学相比，需要特定的程序。本章描述了在Tissue - Tek O.C.T.复合物和石蜡包埋切片上进行免疫组织化学以检测牙齿矿化组织中抗原的方法。