釉原蛋白和成釉蛋白在发育中的釉质基质中的免疫组织化学共定位

Immunohistochemical Co-Localization of Amelogenin and Ameloblastin in Developing Enamel Matrix.

作者信息

Bapat Rucha Arun, Moradian-Oldak Janet

机构信息

Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, USA.

Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, USA.

出版信息

Methods Mol Biol. 2019;1922:219-228. doi: 10.1007/978-1-4939-9012-2_22.

DOI:10.1007/978-1-4939-9012-2_22

PMID:30838580

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8011542/

Abstract

Quantitative co-localization analysis, combined with other in vivo and in vitro techniques, can provide valuable information about the interaction and cooperative function of two proteins. Here we describe in detail the technique of quantitative co-localization analysis of two enamel matrix proteins, amelogenin and ameloblastin, in developing mouse enamel.

摘要

定量共定位分析与其他体内和体外技术相结合，可以提供有关两种蛋白质相互作用和协同功能的有价值信息。在这里，我们详细描述了在发育中的小鼠牙釉质中对两种牙釉质基质蛋白——釉原蛋白和成釉蛋白进行定量共定位分析的技术。