Hüttner Sören S, Ahrens Hellen E, Schmidt Manuel, Henze Henriette, Jung Marie Juliane, Schüler Svenja C, von Maltzahn Julia
Leibniz-Institute on Aging - Fritz-Lipmann-Institute, Jena, Germany.
Methods Mol Biol. 2019;2045:25-36. doi: 10.1007/7651_2019_209.
The isolation and culture of single floating myofibers with their adjacent muscle stem cells allow the analysis and comparison of muscle stem cells from aged and young mice. This method has the advantage that muscle stem cells are cultured on the myofiber, thereby culturing them in conditions as close to their endogenous niche as possible. Here we describe the isolation, culture, transfection with siRNA, and subsequent immunostaining for muscle stem cells on their adjacent myofibers from aged and young mice.
分离并培养带有相邻肌肉干细胞的单个漂浮肌纤维，能够对老年和年轻小鼠的肌肉干细胞进行分析和比较。该方法的优势在于，肌肉干细胞是在肌纤维上进行培养的，从而使其培养条件尽可能接近其内生微环境。在此，我们描述了从老年和年轻小鼠的相邻肌纤维中分离、培养肌肉干细胞，用小干扰RNA（siRNA）转染，以及随后进行免疫染色的方法。
