Jaramillo Alejandra Mejia, Aubin Carl-Éric, Hachem Bahe, Londono Irene, Pelletier Juliette, Parent Stefan, Villemure Isabelle
Polytechnique Montréal, Canada.
J Musculoskelet Neuronal Interact. 2019 Mar 1;19(1):13-20.
To characterize growth plate histology of porcine spines instrumented with a new intra-vertebral staple.
Spinal segments (T7-T9) previously instrumented with an intra-vertebral staple (experimental group, n=7) or non-instrumented (control group, n=4) underwent average growth rate (AGR), and histomorphometric measurements: heights of proliferative (PZH) and hypertrophic (HZH) growth plate zones, hypertrophic cells height (CH), and the number of proliferative chondrocytes per column (CC). These measurements were done over three regions: (1) left side; (2) middle; (3) right side (instrumented side). The two groups were analyzed by comparing the difference between results for regions 1 and 3 (Dif-R1R3).
A significantly higher Dif-R1R3 was found for AGR and HZH for the experimental group as compared with controls. This Dif-R1R3 was also significantly higher for CC at T8 level, CH at T7 level and PZH at both levels. No significant changes for the Dif-R1R3 were observed in the adjacent vertebrae (T11-T12).
This study confirmed the local growth modulation capacity of the intra-vertebral staple, translated at the histomorphometric level by a significant reduction in all parameters, but not in all spinal levels. Further analyses are needed to confirm the regional effect, especially for the intervertebral disc and other connective tissues.
描述使用新型椎体内钉固定的猪脊柱生长板组织学特征。
对先前使用椎体内钉固定的脊柱节段（T7-T9，实验组，n=7）或未固定的脊柱节段（对照组，n=4）进行平均生长速率（AGR）及组织形态计量学测量：增殖区（PZH）和肥大区（HZH）生长板高度、肥大细胞高度（CH）以及每列增殖软骨细胞数量（CC）。这些测量在三个区域进行：（1）左侧；（2）中间；（3）右侧（固定侧）。通过比较区域1和区域3结果的差异（Dif-R1R3）对两组进行分析。
与对照组相比，实验组的AGR和HZH的Dif-R1R3显著更高。在T8水平的CC、T7水平的CH以及两个水平的PZH方面，该Dif-R1R3也显著更高。在相邻椎体（T11-T12）中未观察到Dif-R1R3有显著变化。
本研究证实了椎体内钉的局部生长调节能力，在组织形态计量学水平上表现为所有参数均显著降低，但并非在所有脊柱节段。需要进一步分析以确认区域效应，特别是对椎间盘和其他结缔组织的影响。
