Yang Danting, Zhou Haibo, Dina Nicoleta E, Haisch Christoph
Department of Preventative Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medical School of Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang Province 315211, People's Republic of China.
Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic of Traditional Chinese Medicine and New Drug Research, Jinan University, Guangzhou, Guangdong Province 510632, People's Republic of China.
R Soc Open Sci. 2018 Sep 5;5(9):180955. doi: 10.1098/rsos.180955. eCollection 2018 Sep.
Acute urinary tract infections (UTIs) are one of the most common nosocomial bacterial infections, which affect almost 50% of the population at least once in their lifetime. UTIs may lead to lethal consequences if they are left undiagnosed and not properly treated. Early, rapid and accurate uropathogens detection methods play a pivotal role in clinical process. In this work, a portable bacteria-grasping surface-enhanced Raman scattering (SERS) chip for identification of three species of uropathogens ( CFT 073, PAO1 and PRM1) directly from culture matrix was reported. The chip was firstly modified with a positively charged NH group, which enables itself grasp the negatively charged bacterial cells through the electrostatic adsorption principle. After the bacterial cells were captured by the chip, concentrated Ag nanoparticles (NPs) were used to obtain their Raman fingerprint spectra with recognizable characteristic peaks and good reproducibility. With the help of chemometric method such as discriminant analysis (DA), the SERS-based chip allows a rapid, successful identification of three species of UTI bacteria with a minimal bacterial concentration (10 cells ml) required for clinical diagnostics. In addition, this chip could spot the bacterial SERS fingerprints information directly from LB culture medium and artificial urine without sample pre-treatment. The portable bacteria-grasping SERS-based chip provides a possibility for fast and easy detection of uropathogens, and viability of future development in healthcare applications.
急性尿路感染（UTIs）是最常见的医院获得性细菌感染之一，几乎50%的人一生中至少会感染一次。如果不进行诊断和适当治疗，UTIs可能会导致致命后果。早期、快速且准确的尿路病原体检测方法在临床过程中起着关键作用。在这项工作中，报道了一种便携式细菌捕获表面增强拉曼散射（SERS）芯片，用于直接从培养基质中鉴定三种尿路病原体（CFT 073、PAO1和PRM1）。该芯片首先用带正电荷的NH基团进行修饰，使其能够通过静电吸附原理捕获带负电荷的细菌细胞。在细菌细胞被芯片捕获后，使用浓缩的银纳米颗粒（NPs）获得具有可识别特征峰且重现性良好的拉曼指纹光谱。借助判别分析（DA）等化学计量学方法，基于SERS的芯片能够以临床诊断所需的最低细菌浓度（10个细胞/毫升）快速、成功地鉴定三种UTI细菌。此外，该芯片无需样品预处理即可直接从LB培养基和人工尿液中获取细菌的SERS指纹信息。这种便携式细菌捕获SERS芯片为快速、简便地检测尿路病原体提供了可能，具有在医疗保健应用中未来发展的可行性。
