Bouvet J P, Couderc J, Parlebas J, Pirès R, Duclos H, Pillot J
C R Acad Sci III. 1986;302(7):233-8.
The boar seminal fluid was fractionated in order to purify semen immunosuppressive factors. This species was chosen for its extremely abundant semen which allows large scale purifications and analyses. The fractions were tested on Balb/c mice spleen cells stimulated with LPS or PHA. The most active fraction was found in the 100-110 kd molecular weight range. The immunosuppressive activity occurred on both B and T cells, but was higher on the former. The active molecule is likely to be a protein. Another fraction of greater than 500 kd MW was also immunosuppressive but its activity disappeared by concentration. It possibly contained aggregates of the 100 kd molecules. The biological role of these immunosuppressive agents could be to protect sperm against female local immune reactions. Similar molecules in human semen might exist and favor the occurrence of acquired immunodeficiency syndrome in male homosexuals.
为了纯化精液免疫抑制因子，对公猪精液进行了分级分离。选择该物种是因为其精液极其丰富，便于进行大规模的纯化和分析。将这些分级分离物在经脂多糖（LPS）或植物血凝素（PHA）刺激的Balb/c小鼠脾细胞上进行测试。发现活性最强的分级分离物在分子量100 - 110kd范围内。免疫抑制活性在B细胞和T细胞上均有体现，但对前者的活性更高。活性分子可能是一种蛋白质。另一个分子量大于500kd的分级分离物也具有免疫抑制作用，但其活性在浓缩时消失。它可能包含100kd分子的聚集体。这些免疫抑制剂的生物学作用可能是保护精子免受雌性局部免疫反应的影响。人类精液中可能存在类似分子，并且有利于男性同性恋者获得性免疫缺陷综合征的发生。
