两种用于检测阴道毛滴虫的聚合酶链反应检测方法的比较。
Comparison of Two PCR Assays for Trichomonas vaginalis.
作者信息
Noh Chang-Suk, Kim Sang-Su, Park Sung-Yul, Moon Hong-Sang, Hong Yeonchul, Ryu Jae-Sook
机构信息
Department of Internal Medicine, Hallym University Hangang Sacred Heart Hospital, Seoul 07247, Korea.
Department of Environmental Biology and Medical Parasitology, Hanyang University College of Medicine, Seoul 04763, Korea.
出版信息
Korean J Parasitol. 2019 Feb;57(1):27-31. doi: 10.3347/kjp.2019.57.1.27. Epub 2019 Feb 26.
PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.
已知聚合酶链反应（PCR）是诊断阴道毛滴虫感染最敏感的方法。本研究旨在比较汉阳大学开发的用于滴虫病的PCR检测法（HY-PCR）与使用Seeplex Ace检测试剂盒®的灵敏度，使用从四名患有前列腺疾病的韩国男性收集的尿液。总体而言，HY-PCR比Seeplex试剂盒更敏感。建议使用Chelex 100进行DNA分离，以提高PCR检测的灵敏度。
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