Pernice W, Scherer R, Lüben G, Sodomann C P, Sedlacek H H
Dtsch Med Wochenschr. 1978 Sep 15;103(37):1448-53. doi: 10.1055/s-0028-1129280.
Baby hamster kidney cells fixed in acetone on glass slides were used as antigen for demonstration of antinuclear antibodies. Where certain storage conditions were observed (drying agent, 4 degrees C) they have kept for 12 months up to now. As regards specificity, sensitivity, reproducibility, and differentiation of fluorescent types the baby hamster kidney cell test appears superior to other immunofluorescence methods used (chicken erythrocytes, rat liver sections, and crithidiae). These results were obtained in 73 sera from patients with disseminated lupus erythematodes, drug-induced lupus erythematodes, discoid erythematodes, allergic vasculitis, progressive scleroderma, dermatomyositis, and 36 control sera.
将固定在载玻片上的丙酮中的幼仓鼠肾细胞用作抗原,以检测抗核抗体。在遵守特定储存条件(干燥剂,4摄氏度)的情况下,它们至今已保存了12个月。就荧光类型的特异性、敏感性、可重复性和区分性而言,幼仓鼠肾细胞检测似乎优于所使用的其他免疫荧光方法(鸡红细胞、大鼠肝脏切片和短膜虫)。这些结果来自73份弥漫性红斑狼疮、药物性红斑狼疮、盘状红斑狼疮、过敏性血管炎、进行性硬皮病、皮肌炎患者的血清以及36份对照血清。