相分离固定与水溶液固定的组织学比较。

A histological comparison of phase-partition fixation with fixation in aqueous solutions.

作者信息

Nettleton G S, McAuliffe W G

出版信息

J Histochem Cytochem. 1986 Jun;34(6):795-800. doi: 10.1177/34.6.3084627.

DOI:10.1177/34.6.3084627

Abstract

In phase-partition fixation, tissue is immersed in a non-aqueous solvent at equilibrium with an aqueous solution of a fixing agent to minimize osmotic effects. Preservation of morphology afforded by phase-partition fixation using formalin and glutaraldehyde and several organic solvents was compared to aqueous 10% neutral buffered formalin fixation for five tissues. It was shown that phase-partition fixation can provide excellent fixation for light microscopy if the proper combinations of fixatives and solvents are used.

摘要

在相分离固定中，将组织浸入与固定剂水溶液处于平衡状态的非水溶剂中，以尽量减少渗透效应。将使用福尔马林、戊二醛和几种有机溶剂进行相分离固定所提供的形态保存效果，与五种组织的10%中性缓冲福尔马林水溶液固定效果进行了比较。结果表明，如果使用适当的固定剂和溶剂组合，相分离固定可为光学显微镜提供出色的固定效果。