Bakaruddin Noor Hazwani, Ab Majid Abdul Hafiz
Household and Structural Urban Entomology Laboratory, Vector Control Research Unit, School of Biological Sciences, Universiti Sains Malaysia, 11800 USM Pulau Pinang, Malaysia.
Trop Life Sci Res. 2019 Jan;30(1):33-56. doi: 10.21315/tlsr2019.30.1.3. Epub 2019 Jan 31.
This study examined the tunneling activity and the behaviour of two subterranean termites, and treated with four plant extracts, namely and . All plants were extracted with three different solvents (methanol, hexane and water) and were diluted into three concentrations (500, 5,000 and 10,000 ppm). A group of 50 workers and 2 soldiers were tested and observed daily during the exposure in order to determine their survivorship and behaviour. Both sections were scored to determine their tunneling activities after seven days. There was a significant difference of tunneling activities of on sand treated with plant extracts (X(2) = 31.790, < 0.0001) with a mean rank of 8.50 for methanolic extracts and 32.50 for both hexane and water extracts. Meanwhile, no significant difference was observed on tunneling activity of on treated sand (X(2) = 2.200, = 0.333) with a mean rank of 20.72 for methanolic extracts, 26.31 for water extracts and 26.47 for hexane extracts. Results showed that plants extracted with methanol demonstrated strong repellent properties with 0 tunneling activity on the treated sand and low survivorship of both termites. Moreover, both termites did not survive (0%) when they were treated with methanolic extracts at 10,000 ppm. They also displayed a different behaviour post-exposure such as avoidance, gradually losing the ability to walk and low feeding consumption. These results indicate that there is a strong termiticidal activity of plants extracted with methanol against and .
本研究检测了两种地下白蚁的打洞活动及行为，并用四种植物提取物，即[提取物名称未给出]和[提取物名称未给出]对其进行处理。所有植物均用三种不同溶剂（甲醇、己烷和水）提取，并稀释成三种浓度（500、5000和10000 ppm）。在暴露期间，每天对一组50只工蚁和2只兵蚁进行测试和观察，以确定它们的存活率和行为。七天后，对两个组进行评分以确定它们的打洞活动。在用植物提取物处理的沙子上，[白蚁种类未给出]的打洞活动存在显著差异（X(2)=31.790，P<0.0001），甲醇提取物的平均秩为8.50，己烷和水提取物的平均秩为32.50。同时，在用处理过的沙子上，[白蚁种类未给出]的打洞活动未观察到显著差异（X(2)=2.200，P=0.333），甲醇提取物的平均秩为20.72，水提取物的平均秩为26.31，己烷提取物的平均秩为26.47。结果表明，用甲醇提取的植物表现出很强的驱避特性，在处理过的沙子上打洞活动为0，两种白蚁的存活率都很低。此外，当用10000 ppm的甲醇提取物处理时，两种白蚁都无法存活（0%）。它们在暴露后还表现出不同的行为，如回避、逐渐失去行走能力和低摄食量。这些结果表明，用甲醇提取的植物对[白蚁种类未给出]和[白蚁种类未给出]具有很强的杀白蚁活性。
