Zhao Shulin
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Pharmacy, Guangxi Normal University, Guilin, China.
Methods Mol Biol. 2019;1972:251-259. doi: 10.1007/978-1-4939-9213-3_18.
Microchip electrophoresis (MCE), regarded as a miniaturized version of capillary electrophoresis (CE), has exhibited prominent advantages in terms of low sample consumption, rapid analysis times, easy operation, efficient resolution of compounds, and increased throughput. This technology has led to more research focus on analysis particularly in hospital settings for clinical diagnostics. However, since the channels in microchip are very small, achieving the desired assay sensitivity on a microfluidic platform remains a challenge. Here, we describe aptamer-based MCE assays for amplification detection of carcinoembryonic antigen (CEA) in human serum.
微芯片电泳（MCE）被视为毛细管电泳（CE）的小型化版本，在低样品消耗、快速分析时间、操作简便、化合物高效分离和高通量方面展现出显著优势。这项技术使得更多研究聚焦于分析，尤其是在医院临床诊断环境中。然而，由于微芯片中的通道非常小，在微流控平台上实现所需的检测灵敏度仍然是一项挑战。在此，我们描述了基于适体的MCE检测方法，用于扩增检测人血清中的癌胚抗原（CEA）。
