Aptamer-Based Microchip Electrophoresis Assays for Amplification Detection of Carcinoembryonic Antigen.

Microchip electrophoresis (MCE), regarded as a miniaturized version of capillary electrophoresis (CE), has exhibited prominent advantages in terms of low sample consumption, rapid analysis times, easy operation, efficient resolution of compounds, and increased throughput. This technology has led to more research focus on analysis particularly in hospital settings for clinical diagnostics. However, since the channels in microchip are very small, achieving the desired assay sensitivity on a microfluidic platform remains a challenge. Here, we describe aptamer-based MCE assays for amplification detection of carcinoembryonic antigen (CEA) in human serum.