Leibniz Institute of Photonic Technology Jena, Jena, Germany.
Department of Biomedical Engineering, University of California Davis, Davis, California.
J Biophotonics. 2019 Jul;12(7):e201800447. doi: 10.1002/jbio.201800447. Epub 2019 Apr 3.
Raman spectroscopy using fiber optic probe combines non-contacted and label-free molecular fingerprinting with high mechanical flexibility for biomedical, clinical and industrial applications. Inherently, fiber optic Raman probes provide information from a single point only, and the acquisition of images is not straightforward. For many applications, it is highly crucial to determine the molecular distribution and provide imaging information of the sample. Here, we propose an approach for Raman imaging using a handheld fiber optic probe, which is built around computer vision-based assessment of positional information and simultaneous acquisition of spectroscopic information. By combining this implementation with real-time data processing and analysis, it is possible to create not only fiber-based Raman imaging but also an augmented chemical reality image of the molecular distribution of the sample surface in real-time. We experimentally demonstrated that using our approach, it is possible to determine and to distinguish borders of different bimolecular compounds in a short time. Because the method can be transferred to other optical probes and other spectroscopic techniques, it is expected that the implementation will have a large impact for clinical, biomedical and industrial applications.
基于光纤探头的拉曼光谱学结合了非接触式和无标记的分子指纹图谱技术，具有较高的机械柔韧性，可应用于生物医学、临床和工业领域。光纤拉曼探头本质上只能提供单点信息，因此获取图像并不简单。对于许多应用来说，确定分子分布并提供样品的成像信息是至关重要的。在这里，我们提出了一种基于手持光纤探头的拉曼成像方法，该方法基于基于计算机视觉的位置信息评估和同时获取光谱信息。通过将这种实现与实时数据处理和分析相结合，不仅可以创建基于光纤的拉曼成像，还可以实时创建样品表面分子分布的增强化学现实图像。我们通过实验证明，使用我们的方法可以在短时间内确定和区分不同双分子化合物的边界。由于该方法可以转移到其他光学探头和其他光谱技术，预计该实现将对临床、生物医学和工业应用产生重大影响。
