Toxicology & Environmental Research and Consulting, The Dow Chemical Company, Midland, Michigan.
Environ Mol Mutagen. 2019 Jul;60(6):559-562. doi: 10.1002/em.22280. Epub 2019 Apr 5.
Cell line-based in vitro testing has been widely used as an important component of the genotoxicity testing battery; however, the use of cell lines is constrained by several limitations, including the genetic drift and variability. A study recently reported in the literature comprehensively examined genomic changes in a large number of cell lines and reported extensive genetic variations within the same cell lines across passage numbers and laboratories, even for single-cell derived subclones. The primary objective of this communication is to raise awareness and stimulate discussion within the genotoxicity testing community of the extent of genetic variability of cell lines in general and how these variables could potentially influence the results and reproducibility of genotoxicity testing. Meanwhile, some recommendations for good cell culture practices are highlighted to mitigate, at least to some extent, the concern about genetic variation. Environ. Mol. Mutagen. 60:559-562, 2019. © 2019 Wiley Periodicals, Inc.
基于细胞系的体外测试已被广泛用作遗传毒性测试组合的重要组成部分；然而，细胞系的使用受到多种限制，包括遗传漂移和变异性。最近在文献中报道的一项研究全面检查了大量细胞系中的基因组变化，并报告了相同细胞系在传代数和实验室之间的广泛遗传变异，即使是单细胞衍生的亚克隆也是如此。本通讯的主要目的是提高遗传毒性测试界对细胞系遗传变异性程度的认识，并激发关于这些变量如何潜在影响遗传毒性测试结果和可重复性的讨论。同时，强调了一些良好的细胞培养实践建议，以减轻（至少在一定程度上）对遗传变异的担忧。环境。分子诱变。60:559-562,2019. Wiley Periodicals, Inc.
