a School of Agricultural Engineering/Henan Collaborative Innovation Center of Water Security for Water Source Region of Mid-line of South-to-North Diversion Project , Nanyang Normal University , Nanyang , Henan , China.
Plant Signal Behav. 2019;14(5):e1588669. doi: 10.1080/15592324.2019.1588669. Epub 2019 Mar 8.
Plants producing sufficient amount of aphid alarm pheromone by expressing (E)-β-Farnesene (EβF) synthase gene may contribute to plant protection by reducing aphid populations. However, terpene biosynthesis varies among plant species and developmental stages. In the present study, volatile headspace analysis of tobacco seedlings with MaβFS1 (an EβF synthase from the Asian peppermint Mentha asiatica) failed to generate EβF. We further targeted MaβFS1 to the tobacco plastid, using a chloroplast targeting sequence, either with or without the AtFPS1 gene for the biosynthesis of the precursor farnesyl diphosphate. When both MaβFS1 and AtFPS1 genes were targeted to the chloroplast, low levels of EβF were detected in stably transformed tobacco seedlings; resulting in specific repellence of the green peach aphid, Myzus persicae. These data indicate that redirecting the EβF biosynthetic pathway from its natural cytosolic location to the chloroplast is a valid strategy. This redirecting strategy may be very useful for other crop plants that do not naturally produce EβF or other repellent volatiles.
通过表达（E）-β-法呢烯（EβF）合酶基因，植物可以产生足够量的蚜虫报警信息素，从而通过降低蚜虫种群数量来促进植物保护。然而，萜类化合物的生物合成在植物物种和发育阶段之间存在差异。在本研究中，用 MaβFS1（来自亚洲薄荷 Mentha asiatica 的 EβF 合酶）对烟草幼苗进行挥发性顶空分析未能产生 EβF。我们进一步将 MaβFS1 靶向到烟草质体，使用质体靶向序列，无论是否有用于前体法呢基二磷酸生物合成的 AtFPS1 基因。当 MaβFS1 和 AtFPS1 基因都被靶向到质体时，在稳定转化的烟草幼苗中检测到低水平的 EβF；导致绿桃蚜 Myzus persicae 具有特异性的驱避性。这些数据表明，将 EβF 生物合成途径从其天然细胞质位置重新定向到质体是一种有效的策略。这种重新定向策略对于那些不天然产生 EβF 或其他驱避性挥发物的其他作物植物可能非常有用。
