从一名由伦敦家族性淀粉样前体蛋白（APP）突变（V717I）导致的早发性阿尔茨海默病患者的皮肤成纤维细胞中生成了两条诱导多能干细胞系（ICGi008 - A和ICGi008 - B）。
Generation of two iPSC lines (ICGi008-A and ICGi008-B) from skin fibroblasts of a patient with early-onset Alzheimer's disease caused by London familial APP mutation (V717I).
作者信息
Grigor'eva E V, Malankhanova T B, Ustyantseva E I, Minina J M, Redina O E, Morozov V V, Shevela A I, Zakian S M, Medvedev S P
机构信息
Federal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; E.N. Meshalkin National medical research center, Ministry of Health of the Russian Federation, Novosibirsk, Russia; Institute of Chemical Biology and Fundamental Medicine, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia.
Federal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; E.N. Meshalkin National medical research center, Ministry of Health of the Russian Federation, Novosibirsk, Russia; Institute of Chemical Biology and Fundamental Medicine, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia.
出版信息
Stem Cell Res. 2019 Apr;36:101415. doi: 10.1016/j.scr.2019.101415. Epub 2019 Mar 2.
The induced pluripotent stem cell (iPSC) lines ICGi008-A and ICGi008-B were generated from dermal fibroblasts using episomal vectors expressing pluripotency factors. Dermal fibroblasts were obtained from a 55 year old male Сaucasian familial Alzheimer's disease (AD) patient carrying heterozygous V717I mutation in the APP gene. The generated iPSC lines maintained the original APP genotype, expressed pluripotency markers, exhibited a normal karyotype and retained the ability to differentiate into cell types of the three germ layers. The iPSC lines will be useful for the study of the AD molecular and cellular mechanisms and drug screening.
诱导多能干细胞系ICGi008 - A和ICGi008 - B是使用表达多能性因子的游离型载体从真皮成纤维细胞中产生的。真皮成纤维细胞取自一名55岁的患有家族性阿尔茨海默病（AD）的男性白种人患者，该患者在APP基因中携带杂合V717I突变。所产生的诱导多能干细胞系保持了原始的APP基因型，表达多能性标志物，具有正常的核型，并保留了分化为三个胚层细胞类型的能力。这些诱导多能干细胞系将有助于阿尔茨海默病分子和细胞机制的研究以及药物筛选。
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