砷超富集植物蜈蚣草对砷、镉、锌吸收及其氧化胁迫的交互作用。
Interactive effects of As, Cd and Zn on their uptake and oxidative stress in As-hyperaccumulator Pteris vittata.
机构信息
Institute of Environmental Remediation and Human Health, Southwest Forestry University, Kunming, 650224, China; Soil and Water Science Department, University of Florida, Gainesville, FL, 32611, USA; Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60800, Pakistan.
Soil and Water Science Department, University of Florida, Gainesville, FL, 32611, USA; Institute of Environmental Sciences and Engineering, National University of Science and Technology, Islamabad, Pakistan.
出版信息
Environ Pollut. 2019 May;248:756-762. doi: 10.1016/j.envpol.2019.02.054. Epub 2019 Feb 25.
The effects of arsenic (As), cadmium (Cd) and zinc (Zn) on each other's uptake and oxidative stress in As-hyperaccumulator Pteris vittata were investigated. P. vittata plants were exposed to 50 μM As, Cd and/or Zn for 15 d in 0.2-strength Hoagland solution. When applied alone, P. vittata accumulated 185 mg kg As, 164 mg kg Cd and 327 mg kg Zn in the fronds. While Cd and Zn did not impact each other's uptake, As affected Cd and Zn uptake. Whereas As decreased Zn uptake, Zn affected As speciation in P. vittata fronds, with more arsenate (AsV) than arsenite (AsIII) being present. At 50 μM As, 75 μM Zn increased As accumulation in P. vittata fronds by 10 folds to 2363 mg kg compared to 50 μM Zn. Although AsV was the predominant As species in all tissues, Cd enhanced AsIII levels in the fronds but increased AsV in the roots. Co-exposure of Cd + Zn elevated oxidative stress basing on thiobarbituric acid reactive substances, HO content, Evans blue dye uptake, membrane injury index and reactive oxygen species (ROS) relative to single metal. By lowering Cd and Zn concentrations in P. vittata fronds, As reduced the associated stress comparative to Cd or Zn treatment. The results enhance our understanding of the mechanisms underlying the interactions between As, Cd and Zn in As-hyperaccumulator P. vittata.
研究了砷（As）、镉（Cd）和锌（Zn）对蜈蚣草（As 超积累植物）吸收和氧化应激的相互影响。将蜈蚣草植株在 0.2 倍 Hoagland 溶液中分别暴露于 50 μM As、Cd 和/或 Zn 中 15 d。单独施用时，蜈蚣草叶片中积累了 185 mg/kg As、164 mg/kg Cd 和 327 mg/kg Zn。虽然 Cd 和 Zn 彼此的吸收不受影响，但 As 会影响 Cd 和 Zn 的吸收。As 降低了 Zn 的吸收，而 Zn 影响了 As 在蜈蚣草叶片中的形态，导致叶片中存在更多的砷酸盐（AsV）而不是亚砷酸盐（AsIII）。在 50 μM As 下，75 μM Zn 使蜈蚣草叶片中 As 的积累增加了 10 倍，达到 2363 mg/kg，而单独施用 50 μM Zn 时为 236 倍。尽管所有组织中均以 AsV 为主，但 Cd 增加了叶片中的 AsIII 水平，但增加了根部的 AsV。与单一金属相比，Cd+Zn 的共同暴露基于硫代巴比妥酸反应物质、HO 含量、伊文思蓝染料摄取、膜损伤指数和活性氧（ROS）水平升高了氧化应激。与 Cd 或 Zn 处理相比，As 通过降低蜈蚣草叶片中 Cd 和 Zn 的浓度减轻了相关的应激。这些结果增强了我们对 As 超积累植物蜈蚣草中 As、Cd 和 Zn 相互作用机制的理解。
Zotero 插件